Effect of Wenhua Juanbi Recipe () on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis.

Abstract

OBJECTIVES:

To study the effect of Wenhua Juanbi Recipe (, WJR) on expression of receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), and tumor necrosis factor receptor superfamily member 14 (TNFRSF14, also known as LIGHT) in rats with collagen-induced arthritis (CIA).

METHODS:

CIA rats were generated by subcutaneous injection of bovine collagen type-II at the tail base. Sixty CIA rats were randomly assigned (10 animals/group) to: model, methotrexate (MTX)-treated (0.78 mg/kg body weight), and WJR-treated (22.9 g/kg) groups. Healthy normal rats (n=10) were used as the normal control. Treatments or saline were administered once daily by oral gavage. Rats were sacrifificed at day 28 post-treatment and knee synovium and peripheral blood serum were collected. Toe swelling degree and expression of RANKL, OPG, and LIGHT were determined by Western blot and immunohistochemistry.

RESULTS:

Compared with the normal group, toe swelling degree was signifificantly increased in the model group (P<0.01). After treatment, toe swelling degree decreased signifificantly in the WJR and MTX groups compared with the model group (P<0.01). Compared with the normal group, expression of RANKL and LIGHT were signifificantly increased and OPG signifificantly decreased in peripheral blood and synovium of the model group (P<0.01). Conversely, RANKL and LIGHT expression were signifificantly reduced and OPG increased in the WJR and MTX groups compared with the model group (P<0.01). No statistically significant difference existed between WJR and MTX groups.

CONCLUSION:

WJR likely acts by reducing RANKL expression and increasing OPG expression, thus inhibiting RANKL/RANK interaction and reducing LIGHT expression, thereby inhibiting osteoclast formation/activation to block bone erosion.

• Authors: Xi-de Liu, Yun-Qing Wang, Long Cai, Li-Hong Ye, Fang Wang, Ying-Ying Feng
• Journal: Chinese journal of integrative medicine (Chin J Integr Med) Vol. 23 Issue 3 Pg. 208-214 (Mar 2017) ISSN: 1672-0415 [Print] China
• PMID: 28004300 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Osteoprotegerin
• RANK Ligand
• Receptors, Tumor Necrosis Factor, Member 14
• wenhua juanbi

Topics

• Animals
• Arthritis, Experimental (drug therapy, metabolism)
• Blotting, Western
• Cattle
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Immunohistochemistry
• Male
• Osteoprotegerin (metabolism)
• RANK Ligand (metabolism)
• Rats, Wistar
• Receptors, Tumor Necrosis Factor, Member 14 (metabolism)
• Synovial Membrane (drug effects, pathology)