The enhanced immune responses induced by Salmonella enteritidis ghosts loaded with Neisseria gonorrhoeae porB against Salmonella in mice.

Abstract

Human health has been seriously endangered by highly prevalent salmonellosis and multidrug-resistant Salmonella strains. Current vaccines suffer from variable immune-protective effects, so more effective ones are needed to control Salmonella infection : Bacterial ghosts have been produced by the expression of lysis gene E from bacteriophage PhiX174 and can be filled with considerable exogenous substances such as DNA or drugs as a novel platform. In this study, Salmonella enteritidis (SE) ghosts were developed and loaded with Neisseria gonorrhoeae porin B (porB) to construct a novel inactive vaccine. Our new studies show that SE ghosts loaded with porB displayed increased production of pro-inflammatory cytokines (IL-1β, IL-6, IL-10 and IL-12p70) in bone marrow-derived dendritic cells (BMDCs), and elicited significantly higher specific systemic and mucosal immune responses to Salmonella than SE ghosts alone. In addition, the novel porB-loaded ghosts conferred higher protective effects on virulent Salmonella challenge. For the first time, we demonstrate that N. gonorrhoeae porB, as a novel adjuvant, can increase the immunogenicity of SE ghosts. Our studies suggested that Salmonella enteritidis ghosts loaded with Neisseria gonorrhoeae porin B might be a useful mucosal Salmonella vaccine candidate for practical use in the future.

• Authors: Hongmei Jiao, Hui Yang, Dan Zhao, Li He, Jin Chen, Guocai Li
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 363 Issue 22 (11 2016) ISSN: 1574-6968 [Electronic] England
• PMID: 27797865 (Publication Type: Journal Article)
• Copyright: © FEMS 2016. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Adjuvants, Immunologic
• Antibodies, Bacterial
• IL10 protein, mouse
• IL1B protein, mouse
• Interleukin-1beta
• Interleukin-6
• Porins
• Salmonella Vaccines
• interleukin-6, mouse
• porin protein, Neisseria
• Interleukin-10
• Interleukin-12

Topics

• Adjuvants, Immunologic (administration & dosage)
• Animals
• Antibodies, Bacterial (immunology)
• Cell Membrane (immunology)
• Dendritic Cells (immunology)
• Drug Resistance, Multiple, Bacterial
• Immunity, Mucosal (immunology)
• Interleukin-10 (biosynthesis)
• Interleukin-12 (biosynthesis)
• Interleukin-1beta (biosynthesis)
• Interleukin-6 (biosynthesis)
• Lymphocyte Activation (immunology)
• Mice
• Mice, Inbred BALB C
• Neisseria gonorrhoeae (immunology)
• Porins (immunology)
• Salmonella Food Poisoning (microbiology, prevention & control)
• Salmonella Infections, Animal (microbiology)
• Salmonella Vaccines (administration & dosage, immunology)
• Salmonella enteritidis (cytology, immunology)
• T-Lymphocytes (immunology)