Inflammatory responses of stromal fibroblasts to inflammatory epithelial cells are involved in the pathogenesis of bovine mastitis.

Abstract

Hypernomic secretion of epithelial cytokines has several effects on stromal cells. The contributions of inflammatory epithelial cells to stromal fibroblasts in bovine mammary glands with mastitis remain poorly understood. Here, we established an inflammatory epithelial cell model of bovine mastitis with gram-negative lipopolysaccharide (LPS) and gram-positive lipoteichoic acid (LTA) bacterial cell wall components. We characterized immune responses of mammary stromal fibroblasts induced by inflammatory epithelial cells. Our results showed that inflammatory epithelial cells affected stromal fibroblast characteristics by increasing inflammatory mediator expression, elevating extracellular matrix protein deposition, decreasing proliferation capacity, and enhancing migration ability. The changes in stromal fibroblast proliferation and migration abilities were mediated by signal molecules, such as WNT signal pathway components. LPS- and LTA-induced inflammatory epithelial cells triggered different immune responses in stromal fibroblasts. Thus, in mastitis, bovine mammary gland stromal fibroblasts were affected by inflammatory epithelial cells and displayed inflammation-specific changes, suggesting that fibroblasts play crucial roles in bovine mastitis.

• Authors: Wenyao Zhang, Xuezhong Li, Tong Xu, Mengru Ma, Yong Zhang, Ming-Qing Gao
• Journal: Experimental cell research (Exp Cell Res) Vol. 349 Issue 1 Pg. 45-52 (Nov 15 2016) ISSN: 1090-2422 [Electronic] United States
• PMID: 27680776 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Extracellular Matrix Proteins
• Inflammation Mediators
• Lipopolysaccharides
• RNA, Messenger
• Teichoic Acids
• Tumor Necrosis Factor-alpha
• lipoteichoic acid

Topics

• Animals
• Cattle
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Coculture Techniques
• Disease Models, Animal
• Epithelial Cells (drug effects, metabolism, pathology)
• Extracellular Matrix Proteins (metabolism)
• Female
• Fibroblasts (drug effects, metabolism, pathology)
• Inflammation (genetics, pathology)
• Inflammation Mediators (metabolism)
• Lipopolysaccharides (pharmacology)
• Mastitis, Bovine (pathology)
• RNA, Messenger (genetics, metabolism)
• Stromal Cells (drug effects, pathology)
• Teichoic Acids (pharmacology)
• Tumor Necrosis Factor-alpha (genetics, metabolism)