Pentraxin 3 is an anti-inflammatory protein associated with lipid-induced interleukin 10 in vitro.

Abstract

UNLABELLED:

Pentraxin 3 (PTX3) is an acute phase protein expressed in response to pro-inflammatory stimuli during atherosclerosis. However, recent findings suggest that PTX3 is a counter-regulatory protein which enhances the anti-inflammatory response.

OBJECTIVE:

Therefore, the capacity of PTX3 to alter the inflammatory milieu following in vitro stimulation of PBMCs with the pro-inflammatory lipid, palmitate, was examined.

METHODS:

PBMCs from 17 healthy male donors were isolated and cultured under four separate conditions; 200μmol/L palmitate, a physiologically relevant concentration of PTX3, in combination (pal+PTX3), and an unstimulated time-course control.

RESULTS:

Palmitate-induced production of the counter-regulatory protein PTX3 was positively associated with the production of the anti-inflammatory cytokine interleukin 10 (IL-10) following in vitro stimulation of human PBMCs. Furthermore, stimulation of PBMCs in vitro with 500pg/mL PTX3 elicited a significantly greater increase in IL-10 production compared to the palmitate stimulated conditions. However, PTX3 stimulation did not result in the production of the pro-inflammatory cytokines IL-1β, IL-6, and tumor necrosis factor alpha, and when combined with palmitate, did not alter the pro-inflammatory milieu from PBMCs in this study.

CONCLUSION:

These findings provide evidence supporting the role of PTX3 as a mediator of the anti-inflammatory response in physiologically relevant conditions, and suggests that PTX3 counter regulates the development of atherosclerosis by enhancing the production of IL-10.

• Authors: Aaron L Slusher, Amanda B Mischo, Edmund O Acevedo
• Journal: Cytokine (Cytokine) Vol. 86 Pg. 36-40 (10 2016) ISSN: 1096-0023 [Electronic] England
• PMID: 27450429 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• Cytokines
• IL10 protein, human
• Lipopolysaccharides
• Serum Amyloid P-Component
• Tumor Necrosis Factor-alpha
• Interleukin-10
• PTX3 protein
• Palmitic Acid
• C-Reactive Protein

Topics

• Adult
• Atherosclerosis (immunology)
• C-Reactive Protein (immunology, metabolism)
• Cells, Cultured
• Cytokines (metabolism)
• Humans
• Inflammation
• Interleukin-10 (immunology, metabolism)
• Leukocytes, Mononuclear (immunology, metabolism)
• Lipopolysaccharides (immunology)
• Male
• Palmitic Acid (pharmacology)
• Serum Amyloid P-Component (immunology, metabolism)
• Tumor Necrosis Factor-alpha (metabolism)