Neutralization of IL-6 and TNF-α ameliorates intestinal permeability in DSS-induced colitis.

Abstract

The cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) have been implicated as important mediators of the inflammatory reaction in patients with intestinal inflammation. The present study was designed to investigate the roles of these cytokines on mucosal barrier function in a mouse model of acute colitis with using anti-cytokine strategies. Mice received 3% dextran sulfate sodium (DSS) in their drinking water for 7days showed morphological alteration of mucosa and increase of intestinal permeability. Administration of IL-6 monoclonal antibody (mAb) or TNF-α mAb significantly attenuated intestinal permeability. IL-6 mAb and TNF-α mAb treatment also effectively suppressed the expression of claudin-2 and myosin light chain kinase (MLCK). Taken together, we indicated that anti-IL-6 and anti-TNF-α therapy prevent intestinal permeability induced by intestinal inflammation.

• Authors: Yong-Tao Xiao, Wei-Hui Yan, Yi Cao, Jun-Kai Yan, Wei Cai
• Journal: Cytokine (Cytokine) Vol. 83 Pg. 189-192 (07 2016) ISSN: 1096-0023 [Electronic] England
• PMID: 27155817 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Monoclonal
• Antibodies, Neutralizing
• Interleukin-6
• Tumor Necrosis Factor-alpha
• interleukin-6, mouse
• Dextran Sulfate

Topics

• Animals
• Antibodies, Monoclonal (pharmacology)
• Antibodies, Neutralizing (pharmacology)
• Colitis (chemically induced, metabolism, pathology)
• Dextran Sulfate (toxicity)
• Interleukin-6 (antagonists & inhibitors, metabolism)
• Intestinal Mucosa (metabolism, pathology)
• Male
• Mice
• Permeability
• Tumor Necrosis Factor-alpha (antagonists & inhibitors, metabolism)