Abstract | OBJECTIVES: To quantify the barrier sterols and image the lipid structures in the matrix of acquired cholesteatoma and compare the distribution with that found in stratum corneum from normal skin, with the goal to resolve their potential influence on cholesteatoma growth. METHODS: High-performance thin-layer chromatography (HPTLC) was used to achieve a quantitative biochemical determination of the sterols. The intercellular lipids were visualized by Coherent Anti-Stokes Raman scattering (CARS) microscopy, which enables label-free imaging of the lipids in intact tissue samples. RESULTS: CONCLUSIONS: The content of cholesterol in the cholesteatoma matrix is significantly different from that in stratum corneum from skin, and we confirm that the main structure of the cholesteatoma resembles very thick stratum corneum.
|
Authors | Bjarne Thorsted, Maria Bloksgaard, Alexandra Groza, Lars Peter Schousboe, Nils J Færgeman, Jens A Sørensen, Viggo Svane-Knudsen, Jonathan R Brewer |
Journal | The Annals of otology, rhinology, and laryngology
(Ann Otol Rhinol Laryngol)
Vol. 125
Issue 8
Pg. 627-33
(Aug 2016)
ISSN: 1943-572X [Electronic] United States |
PMID | 27084586
(Publication Type: Journal Article)
|
Copyright | © The Author(s) 2016. |
Chemical References |
- Lipids
- Sterols
- Cholesterol
|
Topics |
- Cholesteatoma, Middle Ear
(diagnostic imaging, metabolism, pathology)
- Cholesterol
(metabolism)
- Chromatography, High Pressure Liquid
- Extracellular Space
(diagnostic imaging, metabolism)
- Humans
- Lipid Metabolism
- Lipids
- Microscopy
- Skin
(diagnostic imaging, metabolism, pathology)
- Spectrum Analysis, Raman
- Sterols
(metabolism)
|