Airway oxidative stress causes vascular and hepatic inflammation via upregulation of IL-17A in a murine model of allergic asthma.

Abstract

Oxidants are generated in asthmatic airways due to infiltration of inflammatory leukocytes and resident cells in the lung. Reactive oxygen species (ROS) such as hydrogen peroxide and superoxide radical may leak into systemic circulation when generated in uncontrolled manner and may impact vasculature. Our previous studies have shown an association between airway inflammation and systemic inflammation; however so far none has investigated the impact of airway oxidative inflammation on hepatic oxidative stress and Th1/Th2/Th17 cytokine markers in liver/vasculature in a murine model of asthma. Therefore, this study investigated the contribution of oxidative stress encountered in asthmatic airways in modulation of systemic/hepatic Th1/Th2/Th17 cytokines balance and hepatic oxidative stress. Mice were sensitized intraperitoneally with cockroach extract (CE) in the presence of aluminum hydroxide followed by several intranasal (i.n.) challenges with CE. Mice were then assessed for systemic/hepatic inflammation through assessment of Th1/Th2/Th17 cytokines and oxidative stress (iNOS, protein nitrotyrosine, lipid peroxides and myeloperoxidase activity). Challenge with CE led to increased Th2/Th17 cytokines in blood/liver and hepatic oxidative stress. However, only Th17 related pro-inflammatory markers were upregulated by hydrogen peroxide (H2O2) inhalation in vasculature and liver, whereas antioxidant treatment, N-acetyl cysteine (NAC) downregulated them. Hepatic oxidative stress was also upregulated by H2O2 inhalation, whereas NAC attenuated it. Therefore, our study shows that airway oxidative inflammation may contribute to systemic inflammation through upregulation of Th17 immune responses in blood/liver and hepatic oxidative stress. This might predispose these patients to increased risk for the development of cardiovascular disorders.

• Authors: Naif O Al-Harbi, Ahmed Nadeem, Mohammed M Al-Harbi, Mushtaq A Ansari, Shakir D AlSharari, Saleh A Bahashwan, Sabry M Attia, Khaled A Al-Hosaini, Ali R Al Hoshani, Sheikh F Ahmad
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 34 Pg. 173-182 (May 2016) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 26953647 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Allergens
• Interleukin-17
• Nuclear Receptor Subfamily 1, Group F, Member 3
• Reactive Oxygen Species
• Rorc protein, mouse
• STAT3 Transcription Factor
• Hydrogen Peroxide

Topics

• Allergens (immunology)
• Animals
• Asthma (immunology)
• Cockroaches (immunology)
• Disease Models, Animal
• Hepatitis (immunology)
• Humans
• Hydrogen Peroxide (metabolism)
• Interleukin-17 (genetics, metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Nuclear Receptor Subfamily 1, Group F, Member 3 (metabolism)
• Oxidative Stress
• Reactive Oxygen Species (metabolism)
• Respiratory Mucosa (physiology)
• STAT3 Transcription Factor (metabolism)
• Th1 Cells (immunology)
• Th17 Cells (immunology)
• Th2 Cells (immunology)
• Up-Regulation
• Vasculitis (immunology)