Imbalance of Th17/Tregs in rats with smoke inhalation-induced acute lung injury.

Abstract

T helper (Th) 17 cells and CD4(+) CD25(+) regulatory T (Treg) cells are supposed to be critically involved in regulating autoimmune and inflammatory diseases. The aim of this study was to investigate the Th17/Treg pattern in rats with gunpowder smog-induced acute lung injury. Wistar rats were equally randomized to three groups: normal control group, ALI 6 h group (smoke inhalation for 6 h) and ALI 24 h group (smoke inhalation for 24 h). We observed changes in cell counting in bronchoalveolar lavage fluid (BALF), alveolar-capillary membrane permeability and lung tissue pathology. Moreover, rats in ALI 6 h and ALI 24 h group showed increased expression of Th17 cell and related cytokines (IL-17 A, IL-6, TGF-β and IL-23). Meanwhile, Treg prevalence and related cytokines (IL-10, IL-2 and IL-35) were decreased. Consequently, the ratio of Th17/Treg was higher after smoke inhalation. Additionally, Th1 cell decreased while Th2 cell increased at 6 h and 24 h after smoke inhalation. In conclusion, Th17/Treg imbalance exists in rats with smoke inhalation-induced acute lung injury, suggesting its potential role in the pathogenesis of this disease.

• Authors: Fan Zhang, Mian-yang Li, Ya-ting Lan, Cheng-bin Wang
• Journal: Scientific reports (Sci Rep) Vol. 6 Pg. 21348 (Feb 17 2016) ISSN: 2045-2322 [Electronic] England
• PMID: 26884314 (Publication Type: Journal Article)

Chemical References

• Smoke
• Peroxidase

Topics

• Acute Lung Injury (etiology, metabolism, pathology)
• Animals
• Bronchoalveolar Lavage Fluid (cytology)
• Capillary Permeability
• Immunophenotyping
• Leukocyte Count
• Lung (immunology, metabolism, pathology)
• Lymphocyte Count
• Lymphocyte Subsets (immunology, metabolism)
• Peroxidase (metabolism)
• Phenotype
• Rats
• Smoke (adverse effects, analysis)
• Smoke Inhalation Injury (complications, immunology, pathology)
• T-Lymphocytes, Regulatory (immunology, metabolism)
• Th17 Cells (immunology, metabolism)