Abstract | BACKGROUND: Plasma levels of the danger-associated molecular patterns (DAMPs) nuclear DNA (nDNA) and mitochondrial DNA ( mtDNA) have been shown to be related to sepsis mortality. However, the intermediate factors and/or mechanisms contributing to this relation are largely unknown. Our aim was to determine whether plasma levels of nDNA and mtDNA are related to the markers of inflammation, severity of shock, and organ damage in septic shock patients. Moreover, we investigated the relationship between plasma levels of nDNA/ mtDNA and inflammatory cytokines during experimental human endotoxemia, a model of systemic inflammation in humans in vivo mimicking some of the hallmarks of early sepsis. METHODS: Blood was sampled from the onset of septic shock until day 28 in 121 septic shock patients and from 1 h before endotoxin administration until 8 h afterward in 12 healthy volunteers. Plasma concentrations of five cytokines and circulating levels of nDNA and mtDNA were measured, and correlations with shock-related parameters and markers of organ damage were investigated. RESULTS: CONCLUSIONS: Our findings indicate a relationship between plasma nDNA levels and the inflammatory response. Furthermore, nDNA levels are associated with markers of shock and organ damage in septic shock patients. Nevertheless, the correlations found are relatively weak and it remains to be determined whether nDNA is merely a marker or directly involved in the pathophysiology of septic shock.
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Authors | Kim Timmermans, Matthijs Kox, Gert Jan Scheffer, Peter Pickkers |
Journal | Shock (Augusta, Ga.)
(Shock)
Vol. 45
Issue 6
Pg. 607-12
(06 2016)
ISSN: 1540-0514 [Electronic] United States |
PMID | 26717107
(Publication Type: Journal Article, Observational Study)
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Chemical References |
- Biomarkers
- Cytokines
- DNA, Mitochondrial
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Biomarkers
(blood)
- Cytokines
(blood)
- DNA, Mitochondrial
(blood)
- Female
- Healthy Volunteers
(statistics & numerical data)
- Humans
- Inflammation
(blood)
- Male
- Middle Aged
- Predictive Value of Tests
- Prospective Studies
- Sensitivity and Specificity
- Severity of Illness Index
- Shock
(blood)
- Shock, Septic
(blood, diagnosis, mortality)
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