Abstract |
Considering the importance of macrophages as the first line of defense against fungal infection and the different roles played by the two M1- and M2-like polarized macrophages, we decided to evaluate the effects of Paracoccidioides brasiliensis infection on GM-CSF- and M-CSF-induced bone marrow-derived macrophages (BMM) from the A/J and B10.A mouse strains, an established model of resistance/susceptibility to PCM, respectively. Upon differentiation, the generated GM- or M-BMMs were characterized by morphological analyses, gene expression profiles, and cytokines production. Our main results demonstrate that GM-BMMs derived from A/J and B.10 produced high levels of pro- and anti-inflammatory cytokines that may contribute to generate an unbalanced early immune response. In accordance with the literature, the B10.A susceptible mice lineage has an innate tendency to polarize into M1-like phenotype, whereas the opposite phenotype occurs in A/J resistance mice. In this context, our data support that susceptibility and resistance are strongly correlated with M1 and M2 polarization, respectively.
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Authors | Calliandra de Souza Silva, Aldo Henrique Tavares, Marcio Sousa Jeronimo, Yasmin Soares de Lima, Lorena da Silveira Derengowski, Anamélia Lorenzetti Bocca, Ildinete Silva-Pereira |
Journal | Mediators of inflammation
(Mediators Inflamm)
Vol. 2015
Pg. 605450
( 2015)
ISSN: 1466-1861 [Electronic] United States |
PMID | 26543326
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chemokines
- Cytokines
- Macrophage Colony-Stimulating Factor
- Granulocyte-Macrophage Colony-Stimulating Factor
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Topics |
- Animals
- Bone Marrow Cells
(metabolism)
- Cell Adhesion
- Cell Differentiation
- Cells, Cultured
- Chemokines
(metabolism)
- Cytokines
(metabolism)
- Gene Expression Profiling
- Gene Expression Regulation
- Granulocyte-Macrophage Colony-Stimulating Factor
(metabolism)
- Inflammation
- Macrophage Colony-Stimulating Factor
(metabolism)
- Macrophages
(metabolism)
- Male
- Mice
- Paracoccidioides
- Paracoccidioidomycosis
(metabolism)
- Phagocytosis
- Phenotype
- Real-Time Polymerase Chain Reaction
- Signal Transduction
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