Abstract |
Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS), characterized by infiltrating myelin-reactive T lymphocytes and demyelinating lesions. Experimental autoimmune encephalomyelitis (EAE) is the animal model widely utilized to study MS. EAE is mediated by CD4(+) T cells and can be induced in EAE-susceptible mice through immunization with a myelin antigen, such as proteolipid protein 139-151 (PLP139-151) in SJL mice. In this PLP-induced EAE model, autoreactive CD4(+) T cells migrate from peripheral tissues into the CNS where they are reactivated resulting in CNS damage. Th1 and Th17 cells produce the pro-inflammatory cytokines IFNγ and IL-17, respectively, that have been shown to have pathogenic roles in EAE and MS. Anti-inflammatory Th2, IL-4 secreting cells, have been indicated to inhibit EAE exacerbation. However, given the inflammatory environment of EAE, Th2 effector cells are outnumbered by Th1/Th17 cells. Regulatory CD4(+) T cells suppress immune reactions and have been demonstrated to be dysfunctional in MS patients. Opioid growth factor (OGF), chemically termed [Met(5)]- enkephalin, is a negative growth factor that interacts with the OGF receptor. The OGF-OGFr axis can be activated through exogenous administration of OGF or a low dosage of naltrexone (LDN), an opioid antagonist. We have previously demonstrated that modulation of the OGF-OGFr axis results in alleviation from relapse-remitting EAE, and that CNS-infiltrating CD3(+) T cells are diminished with exogenous OGF or intermittent blockade with LDN administration. In this paper, we aimed to determine whether OGF or LDN alter the Th effector responses of CD4(+) T lymphocytes within the CNS in established EAE. We report in these studies that the numbers of CD4(+) T lymphocytes in the CNS of EAE mice are decreased following treatment with OGF for five days but not LDN. However, modulation of the OGF-OGFr axis did not result in changes to CD4(+) Th effector cell responses in the CNS of EAE mice.
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Authors | Leslie A Hammer, Hanspeter Waldner, Ian S Zagon, Patricia J McLaughlin |
Journal | Experimental biology and medicine (Maywood, N.J.)
(Exp Biol Med (Maywood))
Vol. 241
Issue 1
Pg. 71-8
(Jan 2016)
ISSN: 1535-3699 [Electronic] England |
PMID | 26202376
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2016 by the Society for Experimental Biology and Medicine. |
Chemical References |
- Narcotic Antagonists
- Enkephalin, Methionine
- Naltrexone
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Topics |
- Animals
- CD4-Positive T-Lymphocytes
(immunology)
- Central Nervous System
(pathology)
- Disease Models, Animal
- Encephalomyelitis, Autoimmune, Experimental
(pathology)
- Enkephalin, Methionine
(administration & dosage)
- Female
- Mice
- Multiple Sclerosis
(pathology)
- Naltrexone
(administration & dosage)
- Narcotic Antagonists
(administration & dosage)
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