Peripheral blood-derived cytokine gene polymorphisms and metabolic profile in women with polycystic ovary syndrome.

Abstract

BACKGROUND:

The imbalance between proinflammatory and anti-inflammatory pathways plays a role in polycystic ovary syndrome (PCOS) etiology. We aimed to investigate the relationship between polymorphisms of genes encoding inflammation-associated cytokines and the metabolic profile of Brazilian women with PCOS.

DESIGN:

Case-control study.

METHODS:

The study included 196 women - 97 with PCOS (diagnosed based on Rotterdam criteria, 2003) and 99 age-matched, healthy women (controls). It was investigated polymorphisms in cytokines genes from peripheral blood-derived DNA by using PCR.

RESULTS:

The frequencies of alleles, genotypes, and phenotypes were similar between women with PCOS and controls. The GG genotype of the -179C/G polymorphism (IL6) was associated with higher glucose levels, while the GA and AA genotypes of the -1082A/G polymorphism (IL10), CT and TT genotypes of the -819A/T polymorphism (IL10), CA and AA genotypes of the -522A/G (IL10) polymorphism, and TA genotype of the +874T/A polymorphism (IFN-γ) were associated with lower total cholesterol and triglycerides levels. The GA genotype of the -1082A/G polymorphism (IL10) and the CC genotype of the 10T/C polymorphism (TGF-β1) were associated with lower and higher Ferriman indices, respectively, in women with PCOS. The AA genotype of the -1082A/G polymorphism (IL10) was associated with lower glucose levels, while the TC genotype of the 10T/C polymorphism (TGF-β1) was associated with a lower lipid accumulation product index and higher high-density lipoprotein cholesterol levels in the PCOS group.

CONCLUSIONS:

The genetic polymorphisms of cytokines are not associated with PCOS development, but may contribute to common metabolic disorders associated with PCOS.

• Authors: Mirelle O Sóter, Cláudia N Ferreira, Mariana F Sales, Ana L Candido, Fernando M Reis, Kátia S Milagres, Carla Ronda, Ieda O Silva, Marinez O Sousa, Karina B Gomes
• Journal: Cytokine (Cytokine) Vol. 76 Issue 2 Pg. 227-235 (Dec 2015) ISSN: 1096-0023 [Electronic] England
• PMID: 26141422 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Cytokines
• Lipoproteins, HDL

Topics

• Adolescent
• Adult
• Alleles
• Brazil
• Case-Control Studies
• Cytokines (blood, genetics)
• Female
• Genetic Predisposition to Disease
• Genotype
• Humans
• Insulin Resistance
• Lipoproteins, HDL (blood)
• Metabolome
• Middle Aged
• Phenotype
• Polycystic Ovary Syndrome (diagnosis, genetics, immunology, metabolism)
• Polymorphism, Single Nucleotide
• Young Adult