Abstract | OBJECTIVE: DESIGN: We investigated the mechanism of induction and consequences of the mitochondrial permeability transition pore (MPTP) in the pancreas using cell biological methods including confocal microscopy, patch clamp technology and multiple clinically representative disease models. Effects of genetic and pharmacological inhibition of the MPTP were examined in isolated murine and human pancreatic acinar cells, and in hyperstimulation, bile acid, alcoholic and choline-deficient, ethionine-supplemented acute pancreatitis. RESULTS: CONCLUSIONS: This work demonstrates the mechanism and consequences of MPTP opening to be fundamental to multiple forms of acute pancreatitis and validates the MPTP as a drug target for this disease.
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Authors | Rajarshi Mukherjee, Olga A Mareninova, Irina V Odinokova, Wei Huang, John Murphy, Michael Chvanov, Muhammad A Javed, Li Wen, David M Booth, Matthew C Cane, Muhammad Awais, Bruno Gavillet, Rebecca M Pruss, Sophie Schaller, Jeffery D Molkentin, Alexei V Tepikin, Ole H Petersen, Stephen J Pandol, Ilya Gukovsky, David N Criddle, Anna S Gukovskaya, Robert Sutton, NIHR Pancreas Biomedical Research Unit |
Journal | Gut
(Gut)
Vol. 65
Issue 8
Pg. 1333-46
(08 2016)
ISSN: 1468-3288 [Electronic] England |
PMID | 26071131
(Publication Type: Journal Article)
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Copyright | Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/ |
Chemical References |
- Inositol Phosphates
- Mitochondrial Membrane Transport Proteins
- Mitochondrial Permeability Transition Pore
- Mitochondrial Proteins
- inositol trispyrophosphate
- PGAM5 protein, human
- Phosphoprotein Phosphatases
- Calcium
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Topics |
- Acinar Cells
(drug effects, metabolism, pathology)
- Animals
- Autophagy
(drug effects)
- Calcium
(metabolism)
- Cell Culture Techniques
- Disease Models, Animal
- Humans
- Inositol Phosphates
(metabolism, pharmacology)
- Mice
- Mitochondria
(enzymology)
- Mitochondrial Membrane Transport Proteins
(antagonists & inhibitors, metabolism)
- Mitochondrial Permeability Transition Pore
- Mitochondrial Proteins
(metabolism)
- Necrosis
- Pancreas
(drug effects, metabolism, pathology)
- Pancreatitis, Acute Necrotizing
(chemically induced, metabolism, pathology)
- Phosphoprotein Phosphatases
(metabolism)
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