Abstract | BACKGROUND: METHODS: 10 CF patients and 5 healthy volunteers consumed 40 g of FS daily for 4 weeks with safety and tolerability being assessed. Urine was evaluated for systemic oxidative stress and plasma for FS metabolites (enterolignans) and cytokine levels. Buccal swabs were analyzed for gene expression of Nrf2-regulated antioxidant enzymes including Heme Oxygenase-1 (HO-1) and NAD(P)H Quinone Oxidoreductase 1 (NQO1). RESULTS: All subjects completed the study without serious adverse events. Plasma levels of enterolignans were detectable in both healthy controls and CF volunteers. CF patients were stratified based on plasma enterolignan levels after 2 weeks of FS administration into high- (174 to 535 nM ED and 232 to 1841 nM EL) and low- (0 to 32 nM ED and 0 to 40 nM EL) plasma lignan cohorts. The low enterolignan level cohort experienced a statistically significant drop in urinary inflammatory IsoP and plasma TNFα levels, while demonstrating higher average NQO1 mRNA levels in buccal epithelium compared to high- lignan patients. CONCLUSIONS: This pilot study demonstrated that FS is tolerated by CF patients. FS metabolites could be detected in the plasma. Future studies will assess appropriate dosing and target populations for FS, while exploring clinical outcomes. TRIAL REGISTRATION: ClinicalTrials.gov identifier: NCT02014181 .
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Authors | Jason B Turowski, Ralph A Pietrofesa, John A Lawson, Melpo Christofidou-Solomidou, Denis Hadjiliadis |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 15
Pg. 148
(May 13 2015)
ISSN: 1472-6882 [Electronic] England |
PMID | 25963404
(Publication Type: Controlled Clinical Trial, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents
- Antioxidants
- Biomarkers
- Fatty Acids, Omega-3
- Lignans
- Phenols
- Plant Extracts
- Heme Oxygenase-1
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Topics |
- Adolescent
- Adult
- Animals
- Anti-Inflammatory Agents
(pharmacology, therapeutic use)
- Antioxidants
(pharmacology, therapeutic use)
- Biomarkers
(metabolism)
- Cystic Fibrosis
(drug therapy, metabolism, pathology)
- Dietary Supplements
- Fatty Acids, Omega-3
(pharmacology)
- Female
- Flax
(chemistry)
- Heme Oxygenase-1
(metabolism)
- Humans
- Inflammation
(drug therapy, metabolism)
- Lignans
(pharmacology, therapeutic use)
- Lung
(drug effects, metabolism, pathology)
- Lung Diseases
(metabolism)
- Male
- Mice
- Middle Aged
- Oxidative Stress
(drug effects)
- Phenols
(pharmacology)
- Phytotherapy
- Pilot Projects
- Plant Extracts
(pharmacology, therapeutic use)
- Seeds
(chemistry)
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