Abstract |
We have previously shown that naturally occurring as well as acquired Abs against the Mycobacterium tuberculosis heat shock protein (HSP)65 protect against the induction of murine autoimmune inflammatory arthritis. In the present work, we have studied the anti-inflammatory effect of prozumab, a humanized anti-HSP mAb in murine inflammatory arthritis and colitis, and its effects on cytokine secretion. Prozumab was shown to bind to HSP60, the highly conserved mammalian homolog of the bacterial protein, and it was found to be effective in protecting and suppressing autoimmune arthritis in the models of adjuvant arthritis and collagen-induced arthritis in rats and mice, respectively, as well as in acute hapten-mediated colitis and chronic, spontaneous colitis models. Mechanistically, prozumab induces IL-10 secretion from naive human PBMCs and suppresses the secretion of IFN-γ and IL-6 from anti-CD3-activated human PBMCs. These findings make prozumab a promising potential drug for treating human rheumatoid arthritis and inflammatory bowel disease, as well as a wide range of autoimmune inflammatory diseases.
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Authors | Rina Ulmansky, Dorit Landstein, Eli Moallem, Virginie Loeb, Avi Levin, Ronit Meyuhas, Galia Katzavian, Shira Yair, Yaakov Naparstek |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 194
Issue 11
Pg. 5103-9
(Jun 01 2015)
ISSN: 1550-6606 [Electronic] United States |
PMID | 25904550
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 by The American Association of Immunologists, Inc. |
Chemical References |
- Antibodies, Monoclonal
- Antibodies, Monoclonal, Humanized
- Bacterial Proteins
- Chaperonin 60
- IL10 protein, human
- IL6 protein, human
- Interleukin-6
- heat-shock protein 65, Mycobacterium
- Interleukin-10
- Interferon-gamma
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Topics |
- Animals
- Antibodies, Monoclonal
(therapeutic use)
- Antibodies, Monoclonal, Humanized
(therapeutic use)
- Arthritis, Experimental
(drug therapy)
- Bacterial Proteins
(immunology)
- Cell Line, Tumor
- Chaperonin 60
(antagonists & inhibitors, immunology)
- Female
- Humans
- Inflammatory Bowel Diseases
(drug therapy)
- Interferon-gamma
(metabolism)
- Interleukin-10
(biosynthesis, genetics, metabolism)
- Interleukin-6
(metabolism)
- Jurkat Cells
- Leukocytes, Mononuclear
(immunology, metabolism)
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Inbred DBA
- Protein Binding
(immunology)
- Rats
- Rats, Inbred Lew
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