Abstract | INTRODUCTION: Streptococcus (S.) pneumoniae is the most common causative pathogen in community-acquired pneumonia and sepsis. Activated protein C (APC) has been implicated as an important anticoagulant and anti-inflammatory mediator. We here sought to determine the role of the anticoagulant and cytoprotective functions of endogenous APC during pneumonia and sepsis caused by S. pneumoniae. MATERIALS & METHODS: Mice were treated intraperitoneally with monoclonal antibody (mAb) 1609 (which inhibits both anticoagulant and cytoprotective effects of APC), mAb 1591 (which inhibits only the anticoagulant effects of APC) or a control antibody mAb prior to infection with viable S. pneumoniae via the airways (to induce pneumonia) or via the tail vein (to induce primary sepsis). Mice were analyzed at 24 or 48 hours after infection. RESULTS: CONCLUSION:
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Authors | Marcel Schouten, Cornelis van 't Veer, Nadia Poulussen, Joost C M Meijers, Marcel Levi, Charles T Esmon, Tom van der Poll |
Journal | Thrombosis research
(Thromb Res)
Vol. 135
Issue 3
Pg. 537-43
(Mar 2015)
ISSN: 1879-2472 [Electronic] United States |
PMID | 25586194
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ltd. All rights reserved. |
Chemical References |
- Anticoagulants
- Cryoprotective Agents
- Protein C
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Topics |
- Animals
- Anticoagulants
(metabolism)
- Blood Coagulation
- Cryoprotective Agents
(metabolism)
- Enzyme Activation
- Male
- Mice
- Mice, Inbred C57BL
- Pneumonia, Pneumococcal
(blood, complications, diagnosis, metabolism)
- Protein C
(metabolism)
- Sepsis
(blood, complications, diagnosis, metabolism)
- Streptococcus pneumoniae
(isolation & purification)
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