Abstract | OBJECTIVES: METHODS: LPS and ATP were used to stimulate the release of IL-1beta from thioglycollate-elicited macrophages from BALB/c mice. The production of IL-1beta was evaluated by ELISA assay and NALP3, caspase-1, IL-beta mRNA levels were determined by reverse transcription-polymerase chain reaction. RESULTS: In cultured thioglycollate-elicited macrophages, we observed that LPS + ATP greatly enhanced IL-1 beta secretion (6938.00 +/- 83.45; P < 0.05) and the mRNA levels of NALP3, caspase-1 which are two main components of NALP3 inflammasome (60.88 +/- 8.28; 1.31 +/- 0.04, P < 0.05 for both). The macrophages co-cultured with losartan showed low production of IL-1beta (3907.50 +/- 143.61; P < 0.05) and low production of NALP3, caspase-1mRNA (29.82 +/- 6.92; 1.12 +/- 0.05, P < 0.05 for both). Losartan did not reduce IL-1beta mRNA(P > 0.05). CONCLUSIONS: Our results show that the NALP3 inflammasome is up-regulated and activated in the mouse macrophage in response to LPS + ATP stimulation. Losartan is able to suppress the LPS + ATP-induced production of IL-1beta protein. In addition, this effectmay be partially mediated by suppressing NALP3 inflammasome activation.
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Authors | Fang Wang, Ling Huang, Zhang-Zhe Peng, Yi-Ting Tang, Miao-Miao Lu, Yu Peng, Wen-Juan Mel, Lin Wu, Zhao-Hui Mo, Jie Meng, Li-Jian Tao |
Journal | Die Pharmazie
(Pharmazie)
Vol. 69
Issue 9
Pg. 680-4
(Sep 2014)
ISSN: 0031-7144 [Print] Germany |
PMID | 25272939
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiotensin II Type 1 Receptor Blockers
- Carrier Proteins
- DNA, Complementary
- Interleukin-1beta
- Lipopolysaccharides
- NLR Family, Pyrin Domain-Containing 3 Protein
- Nlrp3 protein, mouse
- RNA, Messenger
- Adenosine Triphosphate
- Caspase 1
- Losartan
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Topics |
- Adenosine Triphosphate
(antagonists & inhibitors, pharmacology)
- Angiotensin II Type 1 Receptor Blockers
(pharmacology)
- Animals
- Carrier Proteins
(antagonists & inhibitors, biosynthesis, immunology)
- Caspase 1
(biosynthesis)
- DNA, Complementary
(biosynthesis, genetics)
- Enzyme-Linked Immunosorbent Assay
- Immunity, Cellular
(drug effects)
- Interleukin-1beta
(metabolism)
- Lipopolysaccharides
(antagonists & inhibitors, pharmacology)
- Losartan
(pharmacology)
- Macrophages
(drug effects, metabolism)
- Macrophages, Peritoneal
(drug effects, immunology)
- Male
- Mice
- Mice, Inbred BALB C
- NLR Family, Pyrin Domain-Containing 3 Protein
- RNA, Messenger
(biosynthesis, genetics)
- Real-Time Polymerase Chain Reaction
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