Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines.

Abstract

Naringin has been reported to possess diverse pharmacological properties, including anti-arthritic and anti-inflammatory activities. The aim of the present study was to determine the potential anti-inflammatory effect of naringin in a mouse model of carrageenan-induced pleurisy. A single dose of naringin (40 and 80 mg/kg) was administered per oral (p.o.) 1 h before carrageenan (Cg) administration. Pro- and anti-inflammatory cytokines were analysed in pleural fluid. We also assessed the effects of naringin on the expression levels of iNOS, inducible cyclooxygenase isoform (COX-2), ICAM-1, MIP-2, PGE2, STAT3, TGF-β1, nuclear factor kappa B (NF-κB) and inhibitor of kappa B (IκBα) in lung tissue. The histological examinations revealed anti-inflammatory effect of naringin while Cg group deteriorated. Naringin downregulated Th1 and upregulated Th2 cytokines. Western blot analyses revealed increased protein expression of NF-κB, STAT3 and COX-2 and decreased IκBα in response to Cg treatment, which were reversed by the treatment with naringin. In the Cg group, mRNA expression levels of pro-inflammatory mediators upregulated and anti-inflammatory mediators downregulated. Naringin reversed these actions.

• Authors: Sheikh Fayaz Ahmad, Sabry M Attia, Saleh A Bakheet, Khairy M A Zoheir, Mushtaq Ahmad Ansari, Hesham M Korashy, Hala E Abdel-Hamied, Abdelkader E Ashour, Adel R A Abd-Allah
• Journal: Inflammation (Inflammation) Vol. 38 Issue 2 Pg. 846-57 (Apr 2015) ISSN: 1573-2576 [Electronic] United States
• PMID: 25117567 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Flavanones
• Inflammation Mediators
• NF-kappa B
• STAT3 Transcription Factor
• Stat3 protein, mouse
• Carrageenan
• Chuk protein, mouse
• I-kappa B Kinase
• naringin

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Carrageenan (toxicity)
• Cytokines (antagonists & inhibitors, metabolism)
• Dose-Response Relationship, Drug
• Female
• Flavanones (pharmacology, therapeutic use)
• I-kappa B Kinase (antagonists & inhibitors, metabolism)
• Inflammation Mediators (antagonists & inhibitors, metabolism)
• Mice
• Mice, Inbred BALB C
• NF-kappa B (antagonists & inhibitors, metabolism)
• Pleurisy (metabolism, prevention & control)
• STAT3 Transcription Factor (antagonists & inhibitors, metabolism)