NSAIDs are often ingested to reduce the
pain and improve regeneration of tendon after
tendon injury. Although the effects of
NSAIDs in tendon healing have been reported, the data and conclusions are not consistent. Recently, tendon-derived stem cells (TDSCs) have been isolated from tendon tissues and has been suggested involved in tendon repair. Our study aims to determine the effects of
COX-2 inhibitor (
celecoxib) on the proliferation and tenocytic differentiation of TDSCs. TDSCs were isolated from mice Achilles tendon and exposed to
celecoxib. Cell proliferation rate was investigated at various concentrations (0.1, 1, 10 and 100 μg/ml) of
celecoxib by using hemocytometer. The
mRNA expression of tendon associated
transcription factors, tendon associated
collagens and tendon associated molecules were determined by reverse transcription-polymerase chain reaction. The
protein expression of
Collagen I,
Collagen III, Scleraxis and Tenomodulin were determined by Western blotting. The results showed that
celecoxib has no effects on TDSCs cell proliferation in various concentrations (p>0.05). The levels of most tendon associated
transcription factors, tendon associated
collagens and tendon associated molecules genes expression were significantly decreased in
celecoxib (10 μg/ml) treated group (p<0.05).
Collagen I,
Collagen III, Scleraxis and Tenomodulin
protein expression were also significantly decreased in
celecoxib (10 μg/ml) treated group (p<0.05). In conclusion,
celecoxib inhibits tenocytic differentiation of tendon-derived stem cells but has no effects on cell proliferation.