Abstract | CONTEXT: Monocyte adhesion to endothelial cells is an important initial event in atherosclerosis and is partially mediated by adhesion molecule expression on the cell surface. Although estrogens inhibit atherosclerosis development, effects of coadministered progestogen remain controversial. OBJECTIVE: We examined the effects of progestogen on cytokine-stimulated human umbilical venous endothelial cell (HUVEC) expression of adhesion molecules. DESIGN: In HUVECs, adhesion molecule mRNA levels were measured by real-time PCR. Protein expression was quantified by immunocytochemistry and ELISAs. To mimic the monocyte adherence to endothelial cells, we used a flow chamber system to assess progestogen effects on U937 monocytoid cell adherence to HUVEC monolayers. We also examined the suppression effects of adhesion molecules with small interference RNAs. RESULTS: CONCLUSIONS:
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Authors | Fumitake Ito, Hiroshi Tatsumi, Taisuke Mori, Izumi Suganuma, Yukiko Tanaka, Aya Sasaki, Seiki Matsuo, Koichi Iwasa, Jo Kitawaki |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 99
Issue 6
Pg. 2188-97
(Jun 2014)
ISSN: 1945-7197 [Electronic] United States |
PMID | 24606071
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cell Adhesion Molecules
- Medroxyprogesterone Acetate
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Topics |
- Cell Adhesion Molecules
(genetics, metabolism)
- Cell Communication
(drug effects)
- Cell Culture Techniques
- Cells, Cultured
- Endothelium, Vascular
(cytology, drug effects, metabolism)
- Female
- Gene Expression Regulation
(drug effects)
- Human Umbilical Vein Endothelial Cells
(drug effects, physiology)
- Humans
- Infant, Newborn
- Medroxyprogesterone Acetate
(pharmacology)
- Monocytes
(drug effects, physiology)
- U937 Cells
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