Abstract | BACKGROUND AND PURPOSE: EXPERIMENTAL APPROACH: We compared the effects of CSY0073 with those of azithromycin in experiments on bacterial cultures, Pseudomonas aeruginosa biofilm, lung cells and mice challenged intranasally with P. aeruginosa LPS. KEY RESULTS: In contrast to azithromycin, CSY0073 did not inhibit the growth of P. aeruginosa, Staphylococcus aureus or Haemophilus influenzae and had no effect on an established P. aeruginosa biofilm. Bronchoalveolar lavage (BAL) fluids and lung homogenates collected after the LPS challenge in mice showed that CSY0073 and azithromycin (200 mg·kg(-1), i.p.) decreased neutrophil counts at 24 h and TNF-α, CXCL1 and CXCL2 levels in the BAL fluid after 3 h and IL-6, CXCL2 and IL-1β levels in the lung after 3 h compared with the vehicle. However, only azithromycin reduced IL-1β levels in the lung 24 h post LPS challenge. CSY0073 and azithromycin similarly diminished the production of pro-inflammatory cytokines by macrophages, but not lung epithelial cells, exposed to P. aeruginosa LPS. CONCLUSIONS AND IMPLICATIONS:
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Authors | V Balloy, A Deveaux, D Lebeaux, O Tabary, P le Rouzic, J M Ghigo, P F Busson, P Y Boëlle, J Guez Guez, U Hahn, A Clement, M Chignard, H Corvol, M Burnet, L Guillot |
Journal | British journal of pharmacology
(Br J Pharmacol)
Vol. 171
Issue 7
Pg. 1783-94
(Apr 2014)
ISSN: 1476-5381 [Electronic] England |
PMID | 24417187
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 The British Pharmacological Society. |
Chemical References |
- Anti-Inflammatory Agents
- CSY 0073
- Inflammation Mediators
- Lipopolysaccharides
- Azithromycin
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Azithromycin
(analogs & derivatives, pharmacology)
- Biofilms
(drug effects, growth & development)
- Bronchoalveolar Lavage Fluid
(immunology)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Inflammation Mediators
(metabolism)
- Lipopolysaccharides
- Lung
(drug effects, immunology)
- Mice
- Mice, Inbred C57BL
- Pneumonia
(chemically induced, immunology, prevention & control)
- Pseudomonas aeruginosa
(drug effects, growth & development)
- Time Factors
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