Abstract |
Microglia polarization to the classical M1 activation state is characterized by elevated pro-inflammatory cytokines; however, a full profile has not been generated in the early stages of a sterile inflammatory response recruiting only resident microglia. We characterized the initial M1 state in a hippocampal injury model dependent upon tumor necrosis factor ( TNF) receptor signaling for dentate granule cell death. Twenty-one-day-old CD1 male mice were injected with trimethyltin (TMT 2.3 mg/kg, i.p.) and the hippocampus was examined at an early stage (24-h post-dosing) of neuronal death. Glia activation was assessed using a custom quantitative nuclease protection assay. We report elevated mRNA levels for glia response such as ionizing calcium-binding adapter molecule-1 and glial fibrillary acidic protein (Gfap); Fas, hypoxia inducible factor alpha, complement component 1qb, TNF-related genes (Tnf, Tnfaip3, Tnfrsfla); interleukin-1 alpha, Cd44, chemokine (C-C motif) ligand (Ccl)2, Cc14, integrin alpha M, lipocalin (Lcn2), and secreted phosphoprotein 1 (Spp1). These changes occurred in the absence of changes in matrix metalloproteinase 9 and 12, neural cell adhesion molecule, metabotropic glutamate receptor (Grm)3, and Ly6/ neurotoxin 1 (Lynx1), as well as, a decrease in neurotrophin 3, glutamate receptor subunit epsilon (Grin)-2b, and neurotrophic tyrosine kinase receptor, type 3. The M2 anti-inflammatory marker, transforming growth factor beta-1 (Tgfb1) was elevated. mRNAs associated with early stage of injury-induced neurogenesis including fibroblast growth factor 21 and Mki67 were elevated. In the "non-injured" temporal cortex receiving projections from the hippocampus, Lynx1, Grm3, and Grin2b were decreased and Gfap increased. Formalin fixed- paraffin-embedded tissue did not generate a comparable profile.
|
Authors | C A McPherson, B A Merrick, G J Harry |
Journal | Neurotoxicity research
(Neurotox Res)
Vol. 25
Issue 1
Pg. 45-56
(Jan 2014)
ISSN: 1476-3524 [Electronic] United States |
PMID | 24002884
(Publication Type: Journal Article, Research Support, N.I.H., Intramural)
|
Chemical References |
- Biomarkers
- Cytokines
- RNA, Messenger
- Trimethyltin Compounds
- trimethyltin
|
Topics |
- Animals
- Biomarkers
- Cell Death
- Cytokines
(metabolism)
- Hippocampus
(drug effects, metabolism, pathology)
- Inflammation
(genetics, metabolism)
- Male
- Mice
- Microglia
(drug effects, metabolism)
- RNA, Messenger
(metabolism)
- Temporal Lobe
(drug effects, metabolism)
- Trimethyltin Compounds
(toxicity)
|