Organic chemicals in diesel exhaust particles enhance picryl chloride-induced atopic dermatitis in NC/Nga mice.

Abstract

BACKGROUND:

Diesel exhaust particles (DEP) have been reported to worsen allergic airway inflammation in mice. Recently, the organic chemical components of DEP (DEP-OC) were found to be important contributors to the aggravation of allergic airway inflammation in mice. The purpose of this study was to examine the effects of DEP-OC on atopic dermatitis (AD)-like skin lesions induced by picryl chloride (PiCl) in NC/Nga mice.

METHODS:

DEP were extracted with benzene/ethanol, and the soluble organic fraction formed the DEP-OC. NC/Nga male mice received simultaneous application of DEP-OC and/or PiCl on their ears once a week for 9 or 3 weeks. We evaluated skin lesions by noting scaling, eruption, excoriation, erosion, hemorrhage, pathologic changes, production of cytokines, and IgE level in the serum.

RESULTS:

PiCl application alone produced progressively severe AD-like skin lesions. The application of PiCl plus DEP-OC resulted in a marked worsening of skin lesions in the early stages of AD. Moreover, mast cell counts significantly increased in the subcutaneous tissue. Administration of PiCl combined with DEP-OC resulted in a greater increase in the local expression of interleukin-4, keratinocyte chemoattractant, and neutrophils in subcutaneous tissue compared with PiCl treatment alone. In contrast, the combination treatment produced lower levels of IFN-γ compared with PiCl treatment alone.

CONCLUSIONS:

DEP-OC application to the skin aggravated PiCl-induced AD. This aggravation may be due to activation of the Th2-associated immune responses by the organic chemicals in DEP.

• Authors: Kaori Sadakane, Takamichi Ichinose, Hirohisa Takano, Rie Yanagisawa, Ken-ichiro Inoue, Hiroaki Kawazato, Aiko Yasuda, Kazuichi Hayakawa
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 162 Issue 1 Pg. 7-15 ( 2013) ISSN: 1423-0097 [Electronic] Switzerland
• PMID: 23817207 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 S. Karger AG, Basel.

Chemical References

• Organic Chemicals
• Vehicle Emissions
• Picryl Chloride

Topics

• Animals
• Dermatitis, Atopic (chemically induced, immunology, physiopathology)
• Disease Models, Animal
• Gene Expression Regulation (drug effects)
• Male
• Mast Cells (drug effects)
• Mice
• Organic Chemicals (toxicity)
• Picryl Chloride
• Skin (drug effects)
• Vehicle Emissions (analysis, toxicity)