Natural polyamine inhibits mouse skin inflammation and macrophage activation.

Abstract

OBJECTIVE:

Natural polyamines are some of the most abundant polycationic molecules in eukaryotic cells, regulating gene expression. Polyamines have been reported to possess anti-inflammatory activities in many model inflammation systems. However, there is no report on their role in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced dermal edema.

METHODS:

Mouse ear edema was induced by TPA. Edema biopsies were investigated using H&E staining. Levels of nitric oxide (NO) were determined using the Griess reaction. Tumor necrosis factor α (TNFα) and interleukin (IL)-1β levels in cell supernatants were measured by TNFα and IL-1β ELISA kits.

RESULTS:

Spermidine and spermine caused significant decreases in ear thickness, water content, and neutrophil infiltrations in comparison with negative control (p < 0.05). External polyamines reduced the levels of inflammatory mediators such as NO, TNFα, and IL-1β in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. Spermine had a higher inhibitory effect on the production of cytokines such as IL-1β and TNFα in LPS-stimulated murine macrophages compared to other polyamines.

CONCLUSION:

Our findings clearly demonstrated that polyamines are involved in the anti-inflammatory effect by reducing dermal edema thickness and other inflammatory mediators like NO and cytokines in a dose-dependent manner.

• Authors: Souren Paul, Sun Chul Kang
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 62 Issue 7 Pg. 681-8 (Jul 2013) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 23603994 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Interleukin-1beta
• Tumor Necrosis Factor-alpha
• Spermine
• Nitric Oxide
• Tetradecanoylphorbol Acetate
• Spermidine

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Cell Line
• Edema (chemically induced, drug therapy, immunology)
• Interleukin-1beta (immunology)
• Macrophage Activation (drug effects)
• Macrophages (drug effects, immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Nitric Oxide (immunology)
• Skin (drug effects, immunology, pathology)
• Spermidine (pharmacology, therapeutic use)
• Spermine (pharmacology, therapeutic use)
• Tetradecanoylphorbol Acetate
• Tumor Necrosis Factor-alpha (immunology)