Abstract | BACKGROUND: METHODS: RESULTS: DSS-treated RNase-L⁻/⁻ mice exhibited a significantly higher clinical score, delayed leukocyte infiltration, reduced expression of IFN-β, tumor necrosis factor α, interleukin-1β, and interleukin-18 at early times post-DSS exposure, and increased mortality as compared with wild-type mice. DSS/AOM-treated RNase-L⁻/⁻ mice displayed an increased tumor burden. Bacterial RNA triggered IFN-β production in an RNase-L-dependent manner and provided a potential mechanism by which RNase-L contributes to the gastrointestinal immune response to microbiota and protects against experimental colitis and colitis-associated cancer. CONCLUSIONS:
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Authors | Tiha M Long, Arindam Chakrabarti, Heather J Ezelle, Sarah E Brennan-Laun, Jean-Pierre Raufman, Irina Polyakova, Robert H Silverman, Bret A Hassel |
Journal | Inflammatory bowel diseases
(Inflamm Bowel Dis)
Vol. 19
Issue 6
Pg. 1295-305
(May 2013)
ISSN: 1536-4844 [Electronic] England |
PMID | 23567782
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carcinogens
- Cytokines
- Interferon Type I
- RNA, Messenger
- Dextran Sulfate
- Peroxidase
- Endoribonucleases
- 2-5A-dependent ribonuclease
- Azoxymethane
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Topics |
- Animals
- Azoxymethane
(toxicity)
- Blotting, Western
- Carcinogens
(toxicity)
- Colitis
(chemically induced, complications)
- Colonic Neoplasms
(etiology, metabolism, pathology)
- Cytokines
(genetics, metabolism)
- Dextran Sulfate
(toxicity)
- Disease Models, Animal
- Endoribonucleases
(physiology)
- Enzyme-Linked Immunosorbent Assay
- Female
- Flow Cytometry
- Immunity, Innate
(immunology)
- Immunoenzyme Techniques
- Interferon Type I
(metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Peroxidase
(metabolism)
- RNA, Messenger
(genetics)
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
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