Abstract | BACKGROUND: METHODS: Male Sprague-Dawley rats were subjected to HS (50% blood loss) and randomized into five groups (n = 7-8 per group) as follows: (1) isotonic sodium chloride solution (ISCS), (2) 7.5% saline, (3) VPA, (4) ISCS + VPA, and (5) HTS + VPA. After 24 hours, they underwent CLP, followed by the same doses of ISCS, HTS, and/or VPA and were monitored for 10 days. In a parallel experiment, blood, peritoneal irrigation fluid and lung homogenate were subjected to enzyme-linked immunosorbent assay 3 hours and 24 hours after CLP to measure myeloperoxidase activity and proinflammatory cytokines tumor necrosis factor α and interleukin 1β levels. Western blotting was performed to investigate the expression of pentraxin 3 protein in the lung homogenate at 24 hours after CLP. Hematoxylin and eosin staining of lungs at the 24 hours were performed to quantify the degree of acute lung injury. RESULTS: CONCLUSION: This is the first study to show that VPA and HTS can work synergistically to attenuate inflammation and improve survival in a lethal two-hit model.
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Authors | Zhengcai Liu, Yongqing Li, Baoling Liu, Danielle K Deperalta, Ting Zhao, Wei Chong, Xiuzhen Duan, Peter Zhou, George C Velmahos, Hasan B Alam |
Journal | The journal of trauma and acute care surgery
(J Trauma Acute Care Surg)
Vol. 74
Issue 4
Pg. 991-7; discussion 997-8
(Apr 2013)
ISSN: 2163-0763 [Electronic] United States |
PMID | 23511136
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Cytokines
- Enzyme Inhibitors
- Saline Solution, Hypertonic
- Serum Amyloid P-Component
- PTX3 protein
- Valproic Acid
- C-Reactive Protein
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Topics |
- Acute Lung Injury
(drug therapy, metabolism)
- Animals
- Blotting, Western
- C-Reactive Protein
(metabolism)
- Cytokines
(metabolism)
- Disease Models, Animal
- Drug Synergism
- Drug Therapy, Combination
- Enzyme Inhibitors
(therapeutic use)
- Enzyme-Linked Immunosorbent Assay
- Male
- Rats
- Rats, Sprague-Dawley
- Resuscitation
(methods)
- Saline Solution, Hypertonic
(therapeutic use)
- Serum Amyloid P-Component
(metabolism)
- Valproic Acid
(therapeutic use)
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