Cytokine expression and the role of Thl7 cells in mice colitis.

Abstract

BACKGROUND/AIMS:

To explore the expression and mechanism of Thl7 cells and cytokines in mice model with 2,4,6-trinitrobenzenesulphonic acid (TNBS)-induced inflammatory bowel disease (IBD).

METHODOLOGY:

ELISA assay was used to detect the expression of Th17 cytokine IL-17 and Thl cytokines IFN-y in colon tissues. Western blot assay was applied to detect IL-17 expression in peripheral blood mononuclear cells (PBMC), spleen mononuclear cells (SMC), mesenteric lymph node cells and colon tissue of colitic mice. RT-PCR assay was used to detect the effect of anti-IL-17 antibody application on TNF-a, IFN-y and IL-6 mRNA levels in SMCs from colitic mice.

RESULTS:

Th17 cytokine IL-17 and Thl cytokines IFN-y were both expressed at high level in TNBS-induced colitic mice. In addition, the expression of Thl7 cytokine appeared earlier than the Thl cytokine. IL-17 levels in SMCs, mesenteric lymph node cells and colon tissue of the disease model group had significant differences compared with normal control group (p<0.01), while the IL-17 level in PBMCs of the disease model group had no significant difference (p>0.05) to control group. After application of 10 ug/mL anti-IL-7 antibody, the TNF-ct, IL-6 and IFN-y mRNA levels in SMCs of the model group showed no significant difference from that of no antibody group (p>0.05).

CONCLUSIONS:

Both Th17 cells and Thl cells involved in TNBS-induced IBD and the role of Thl17 cells may be through inducing the secretion of pro-inflammatory cytokines.

• Authors: Ying He, Lian-Jie Lin, Chang-Qing Zheng, Yu Jin, Yan Lin
• Journal: Hepato-gastroenterology (Hepatogastroenterology) Vol. 59 Issue 118 Pg. 1809-13 (Sep 2012) ISSN: 0172-6390 [Print] Greece
• PMID: 23115792 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Cytokines
• Inflammation Mediators
• RNA, Messenger
• Trinitrobenzenesulfonic Acid

Topics

• Animals
• Antibodies (pharmacology)
• Blotting, Western
• Cells, Cultured
• Colitis (blood, chemically induced, genetics, immunology)
• Colon (drug effects, immunology)
• Cytokines (blood, genetics, metabolism)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Female
• Gene Expression Regulation
• Inflammation Mediators (blood, metabolism)
• Leukocytes, Mononuclear (immunology)
• Mice
• Mice, Inbred BALB C
• RNA, Messenger (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Signal Transduction
• Spleen (immunology)
• Th1 Cells (immunology)
• Th17 Cells (drug effects, immunology)
• Time Factors
• Trinitrobenzenesulfonic Acid