Comprehensive analysis of inflammatory immune mediators of the intraocular fluid aspirated from the foldable capsular vitreous body filled-eyes.

Abstract

PURPOSE:

To analyze the level of human inflammatory immune mediators in the intraocular fluid aspirated from foldable capsular vitreous body (FCVB) filled-eyes during FCVB removal surgery, 3 months after implantation.

METHODS:

8 samples of intra-FCVB fluids (n = 8) were collected from 8 FCVB filled patients in our previous FCVB exploratory clinical trial. The intra-FCVB fluids were aspirated from the FCVB filled-eyes during the FCVB removal surgeries at the third month. For the control groups, the vitreous fluids were collected from patients with idiopathic macular hole (n = 9) or rhegmatogenous retinal detachment (n = 6) during pars plana vitrectomy. A multiplex immunoassay was used to determine levels of 9 cytokines (IL-1β, IL-2, IL-6, IL-8, IL-10, IL-17, TNF-α, IFN-γ and VEGF) in these samples. The VEGF level of some intra-FCVB fluids (n = 6) were re-tested using enzyme-linked immunosorbent assay (ELISA).

RESULTS:

In the intra-FCVB fluids, 9 cytokines concentrations of most samples (n = 5) measured by Multiplex immunoassay showed low values, except for Patient 02, 06, and 09. The VEGF concentrations of some intra-FCVB fluids (n = 6) tested by ELISA were in accordance with Multiplex immunoassay results. For all eight patients (n = 8), the concentrations of IL-1β, IL-2, IL-6, TNF-α, IFN-γ and VEGF were slightly higher as compared to the idiopathic macular hole control group. While, the concentrations of IL-8, IL-10, and IL-17 were not statistically significant different compared with the idiopathic macular hole control samples. Most cytokines concentrations (IL-2, IL-6, IL-8, IL-10, IL-17, TNF-α, IFN-γ, VEGF) were not statistically significant different compared to the rhegmatogenous retinal detachment control group except IL-1β.

CONCLUSIONS:

The FCVB had sufficient porosity to allow cytokines to pass through. This study first discovered that the FCVB possesses favorable permeability of proteins in the human eye.

• Authors: Peijuan Wang, Qianying Gao, Xiaofeng Lin, Shaochong Zhang, Jie Hu, Yaqin Liu, Nuo Xu, Jian Ge
• Journal: PloS one (PLoS One) Vol. 7 Issue 10 Pg. e46384 ( 2012) ISSN: 1932-6203 [Electronic] United States
• PMID: 23049699 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Vascular Endothelial Growth Factor A

Topics

• Aqueous Humor (immunology)
• Biopsy, Needle
• Cytokines (analysis)
• Enzyme-Linked Immunosorbent Assay
• Humans
• Immunoassay
• Prostheses and Implants (adverse effects)
• Retinal Detachment (surgery)
• Statistics, Nonparametric
• Vascular Endothelial Growth Factor A (analysis)
• Vitrectomy
• Vitreous Body (chemistry, surgery)