Cell therapy with adipose tissue-derived stem/stromal cells for elastase-induced pulmonary emphysema in rats.

Abstract

AIMS:

The purpose of this study was to elucidate the mechanism underlying the effects of adipose tissue-derived stem/stromal cell (ASC) transplantation on porcine pancreatic elastase-induced emphysema.

MATERIALS & METHODS:

ASCs (2.5 × 10(6)) were transplanted into pancreatic elastase (250 U/kg)-treated rats, after which gas exchange and growth factor/cytokine levels in lung tissue were determined.

RESULTS:

ASC transplantation restored pulmonary function (arterial oxygen tension and alveolar-arterial oxygen tension difference) almost to that of normal animals. Enlargement of the alveolar airspaces was inhibited. HGF and CINC-1 levels were significantly higher in the ASC group even at 2 weeks after transplantation. Sponge implantation with CINC-1 induced neovascular formation with increased HGF. In vitro secretion of HGF and CINC-1 from ASCs was promoted in the presence of IL-1β.

CONCLUSION:

Not only HGF, but also CINC-1, secreted from transplanted and viable ASCs presumably contributed to lung repair through angiogenesis.

• Authors: Naoki Furuya, Mitsuko Takenaga, Yuki Ohta, Yukie Tokura, Akemi Hamaguchi, Aya Sakamaki, Hirotaka Kida, Hiroshi Handa, Hiroki Nishine, Masamichi Mineshita, Teruomi Miyazawa
• Journal: Regenerative medicine (Regen Med) Vol. 7 Issue 4 Pg. 503-12 (Jul 2012) ISSN: 1746-076X [Electronic] England
• PMID: 22817624 (Publication Type: Journal Article)

Chemical References

• Chemokine CXCL1
• Cxcl1 protein, rat
• Cytokines
• Gases
• Intercellular Signaling Peptides and Proteins
• Pancreatic Elastase

Topics

• Adipose Tissue (cytology)
• Animals
• Cell- and Tissue-Based Therapy
• Cells, Cultured
• Chemokine CXCL1 (metabolism)
• Cytokines (metabolism)
• Gases (metabolism)
• Intercellular Signaling Peptides and Proteins (metabolism)
• Lung (metabolism, pathology)
• Male
• Neovascularization, Physiologic
• Pancreatic Elastase
• Prosthesis Implantation
• Pulmonary Emphysema (chemically induced, pathology, therapy)
• Rats
• Stem Cell Transplantation
• Stem Cells (cytology)
• Stromal Cells (transplantation)
• Sus scrofa