Betula platyphylla attenuated mast cell-mediated allergic inflammation in vivo and in vitro.

Abstract

AIMS:

Betula platyphylla (B. platyphylla) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of B. platyphylla is not completely understood. The aim of the present study is to elucidate whether and how B. platyphylla modulates the mast cell-mediated allergy inflammation in vitro and in vivo.

MAIN METHODS:

We investigated to ascertain the pharmacological effects of B. platyphylla on both compound 48/80 or histamine-induced scratching behaviors and 2, 4-dinitrochlrobenzene (DNCB)-induced atopic dermatitis in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of B. platyphylla, we evaluated the effects of B. platyphylla on the release of histamine in compound 48/80-induced rat peritoneal mast cells (RPMCs), production of inflammatory mediators and activation of nuclear factor-κB (NF-κB) and caspase-1 in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1).

KEY FINDINGS:

The finding of this study demonstrated that B. platyphylla reduced compound 48/80 or histamine-induced scratching behaviors and DNFB-induced atopic dermatitis in mice. Additionally, B. platyphylla inhibited the release of histamine in RPMC and production of inflammatory cytokines as well as the activation of NF-κB and caspase-1 in stimulated HMC-1.

SIGNIFICANCE:

Collectively, the findings of this study provide us with novel insights into the pharmacological actions of B. platyphylla as a potential molecule for use in the treatment of allergic inflammatory diseases.

• Authors: Sa-Rang Oh, Jae-Young Um, Hyong-Jun Choi, Chae-Kwang Im, Kwang-Joong Kim, Ji-Wook Jung, Gil-Saeng Jeong, Seung-Heon Hong, Su-Jin Kim
• Journal: Life sciences (Life Sci) Vol. 91 Issue 1-2 Pg. 20-8 (Jul 26 2012) ISSN: 1879-0631 [Electronic] Netherlands
• PMID: 22683427 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012. Published by Elsevier Inc.

Chemical References

• Anti-Inflammatory Agents
• Plant Extracts
• p-Methoxy-N-methylphenethylamine

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, pharmacology, therapeutic use)
• Betula (chemistry)
• Blotting, Western
• Cell Line
• Dermatitis, Atopic (chemically induced, drug therapy)
• Humans
• Male
• Mast Cells (drug effects, immunology)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred ICR
• Plant Extracts (chemistry, pharmacology, therapeutic use)
• Plant Roots (chemistry)
• Rats
• Republic of Korea
• p-Methoxy-N-methylphenethylamine (pharmacology)