IFN-λ inhibits HIV-1 integration and post-transcriptional events in vitro, but there is only limited in vivo repression of viral production.

Abstract

The lambda interferons (IL-28a, 28b, and IL-29) inhibit the replication of many viruses, but their role in the inhibition of HIV-1 infection remains unclear. During this study, we monitored IL-29 production in HIV-1 infected individuals and analyzed the in vitro and in vivo inhibition of HIV-1 production. Prior treatment with IL-28a or IL-29 induced an antiviral state in cultured primary T-cells, which suppressed HIV-1 integration and post-transcriptional events. The antiviral factors MxA, OAS, and PKR were up-regulated. In HIV-1 infected patients, IL-29 level was increased along with the depletion of CD4⁺ T-cells in peripheral blood, while the elevated IL-29 did not show a significantly negative correlation with viral load. Further analysis of HIV-1 infected individuals showed that IL-29 was positively correlated with IFN-β and anti-inflammatory cytokine IL-10, and was negatively correlated with IFN-γ, which might suggest that IFN-λ participates in modulating antiviral immune responses during HIV-1 infection in vivo. Together, although IFN-λ impeded HIV-1 infection of T-cells in vitro, IFN-λ showed only limited in vivo repression of viral production. The modulation of IFN-λ on inflammatory factors might be worthy for further concentrating on for better understanding the host immune response during HIV-1 infection.

• Authors: Ren-Rong Tian, Hong-Xiong Guo, Ji-Fu Wei, Chuan-Kun Yang, Shao-Heng He, Jian-Hua Wang
• Journal: Antiviral research (Antiviral Res) Vol. 95 Issue 1 Pg. 57-65 (Jul 2012) ISSN: 1872-9096 [Electronic] Netherlands
• PMID: 22584351 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• interferon-lambda, human
• Interleukins
• Interleukin-10
• Interferon-beta
• Interferon-gamma
• Interferons

Topics

• CD4 Lymphocyte Count
• Cells, Cultured
• Gene Expression Profiling
• Gene Expression Regulation
• HIV-1 (growth & development, immunology, isolation & purification, physiology)
• Humans
• Interferon-beta (blood)
• Interferon-gamma (blood)
• Interferons
• Interleukin-10 (blood)
• Interleukins (blood, immunology)
• T-Lymphocytes (immunology, virology)
• Viral Load
• Virus Integration (immunology)