Expression of a murine homolog of apoptosis-inducing human IL-24/MDA-7 in murine tumors fails to induce apoptosis or produce anti-tumor effects.

Abstract

Expression of human interleukin (IL)-24 in tumors achieved anti-tumor effects through apoptosis. IL-24 also induced secretion of proinflammatory cytokines, suggesting the role in immunity. We showed that murine IL-24 transcripts started from the second initiation codon and that expressed mIL-24 in tumors failed to induce apoptosis. Proliferation of murine cells expressing mIL-24 was the same as that of the parent cells and inoculation of the mIL-24-expressing tumors into syngeneic mice did not produce anti-tumor effects. Secretory mIL-24 did not induce the expression of the IL-6, TNF-α or IFN-γ gene in spleen cells. Expression of mIL-24 receptor subunits, IL-22R and IL-20R1, was undetectable in spleen cells even though they were stimulated by anti-CD3, anti-CD40 antibody or concanavalin A. Transduction of murine tumors with adenoviruses expressing the human IL-24 gene however suppressed the viability and decreased the tumor growth. These data suggest that mIL-24 is functionally irrelevant to the human counterpart.

• Authors: Hiroyasu Nagakawa, Osamu Shimozato, Ling Yu, Akihiko Wada, Kiyoko Kawamura, Quanhai Li, Sunil Chada, Yuji Tada, Yuichi Takiguchi, Koichiro Tatsumi, Masatoshi Tagawa
• Journal: Cellular immunology (Cell Immunol) 2012 Jan-Feb Vol. 275 Issue 1-2 Pg. 90-7 ISSN: 1090-2163 [Electronic] Netherlands
• PMID: 22475191 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Cytokines
• Il24 protein, mouse
• Interleukins
• interleukin-24

Topics

• Amino Acid Sequence
• Animals
• Apoptosis
• Cell Line
• Cytokines (chemistry, genetics, immunology)
• Female
• Gene Expression Regulation
• Humans
• Interleukins (genetics, immunology)
• Mice
• Molecular Sequence Data
• Neoplasms (genetics, immunology, pathology)
• Sequence Alignment