Abstract | BACKGROUND: Animal experiments have suggested that a high intraperitoneal pressure ( IPP) might adversely affect the surgical peritoneal environment. The present experimental study investigates the impact of IPP of a CO(2) pneumoperitoneum on human peritoneum. METHODS: Patients undergoing laparoscopic surgery were subjected to either low (8 mmHg) or standard (12 mmHg) IPP. Normal peritoneum was collected from the parietal wall at the beginning of surgery and every 60 min thereafter. Expression levels of 168 genes that encode extracellular matrix proteins, adhesion molecules or inflammatory cytokine signaling molecules were measured in peritoneal tissues using real-time polymerase chain reaction (PCR)-based assay panels. Human peritoneal mesothelial cells (HPMCs) and human peritoneal fibroblasts (HPFBs) were incubated in a CO(2) insufflation chamber for 1 h at 12 or 8 mmHg. Hyaluronan (HA) synthesis and mRNA expression levels of hyaluronic acid synthases (HAS) and hyaluronidases (Hyal) in HPMCs and HPFBs were measured at 0, 4, 8, 12, 24 and 48 h after CO(2) gas exposure by ELISA and real-time PCR, respectively. RESULTS: CONCLUSIONS: A low IPP (8 mmHg) may be better than the standard IPP (12 mmHg) to minimize the adverse impact on the surgical peritoneal environment during a CO(2) pneumoperitoneum.
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Authors | Sachiko Matsuzaki, Kris Jardon, Elodie Maleysson, Francis D'Arpiany, Michel Canis, Revaz Botchorishvili |
Journal | Human reproduction (Oxford, England)
(Hum Reprod)
Vol. 27
Issue 6
Pg. 1613-23
(Jun 2012)
ISSN: 1460-2350 [Electronic] England |
PMID | 22454461
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cell Adhesion Molecules
- Cytokines
- Extracellular Matrix Proteins
- Carbon Dioxide
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Topics |
- Adult
- Aged
- Carbon Dioxide
- Cell Adhesion Molecules
(genetics)
- Cytokines
(genetics)
- Extracellular Matrix Proteins
(genetics)
- Female
- Gene Expression Profiling
- Humans
- Hysterectomy
(methods)
- Laparoscopy
(methods)
- Middle Aged
- Peritoneum
(physiology)
- Pneumoperitoneum, Artificial
(adverse effects, methods)
- Pressure
(adverse effects)
- Real-Time Polymerase Chain Reaction
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