Abstract | BACKGROUND: OBJECTIVES: Evaluation of VEGF expression and production, nitric oxide (NO) production, iNOS expression, and the antioxidant response of mesenchymal stem cells (MSCs), both before and after 12 weeks of treatment with the TNF-α inhibitors adalimumab or etanercept. METHODS: Biochemical, morphological and immunohistochemical analyses were performed in MSCs isolated from nonlesional, perilesional and lesional skin of patients with psoriasis, before and after treatment. RESULTS: The treatments were able to reduce the expression and production of VEGF, the expression of iNOS and the production of NO in MSCs of patients with psoriasis. TNF-α inhibitors also reduced the oxidative damage in MSC membrane and proteins, several antioxidant systems responded to treatments with a general inhibition of activities ( glutathione S-transferase and catalase) and these effects were also supported by a general decrease of total oxyradical scavenging capacity towards hydroxyl radicals and peroxynitrite. CONCLUSIONS: TNF-α inhibitors are able to change the physiopathological pathway of psoriasis, and our results suggest their therapeutic effects already take place at the level of MSCs, which probably represent the cells primarily involved in the 'psoriatic march'.
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Authors | A Campanati, M Orciani, S Gorbi, F Regoli, R Di Primio, A Offidani |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 167
Issue 1
Pg. 68-76
(Jul 2012)
ISSN: 1365-2133 [Electronic] England |
PMID | 22356229
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2012 The Authors. BJD © 2012 British Association of Dermatologists. |
Chemical References |
- Antibodies, Monoclonal, Humanized
- Immunoglobulin G
- Receptors, Tumor Necrosis Factor
- Tumor Necrosis Factor-alpha
- Vascular Endothelial Growth Factor A
- Nitric Oxide
- Nitric Oxide Synthase Type II
- Adalimumab
- Etanercept
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Topics |
- Adalimumab
- Antibodies, Monoclonal, Humanized
(therapeutic use)
- Biological Therapy
(methods)
- Case-Control Studies
- Etanercept
- Female
- Flow Cytometry
- Humans
- Immunoglobulin G
(therapeutic use)
- Immunohistochemistry
- Male
- Mesenchymal Stem Cells
(metabolism)
- Middle Aged
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase Type II
(biosynthesis)
- Psoriasis
(drug therapy, pathology)
- Receptors, Tumor Necrosis Factor
(therapeutic use)
- Tumor Necrosis Factor-alpha
(antagonists & inhibitors)
- Vascular Endothelial Growth Factor A
(biosynthesis, metabolism)
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