METHODS: An orthotopic
transplantation model of murine forestomach
carcinoma was established using the 615 mouse line. The mice bearing
tumors were randomly divided into four groups (30 mice each group):
anesthesia alone,
laparotomy, mini-
laparotomy, and CO(2) insufflation. After the operation, peritoneal macrophages were collected from 6 mice in each group and cultured. The phagocytosis of
neutral red by macrophages and the levels of NO, TNF-α,
IL-10, and
VEGF produced by macrophages were measured after 12, 24, 48, and 72 h of culture. The remaining mice were observed after 2 weeks for the rate of peritoneal
metastasis of forestomach
carcinoma cells and the total weight of implanted nodules.
RESULTS: In the
laparotomy group, 4 mice died intraoperatively and 2 died in the CO(2) insufflation group. The uptake of
neutral red by peritoneal macrophages and the levels of NO, TNF-α,
IL-10, and
VEGF secreted by peritoneal macrophages in the
laparotomy group and mini-
laparotomy group after 12 h of culture were all significantly higher than those in the
anesthesia-alone group (p < 0.05). The corresponding levels in the CO(2) insufflation group after 12 h were all significantly lower than those in the
anesthesia-alone group (p < 0.05). There were no significant differences among the four groups at 24, 48, and 72 h after culture. Comparing with those in the
laparotomy group, the uptake of
neutral red by peritoneal macrophages and the levels of NO, TNF-α,
IL-10, and
VEGF secreted by peritoneal macrophages in the CO(2) insufflation group were all significantly lower after 12 h of culture (p < 0.05), but did not differ significantly at 24, 48, and 72 h of culture (p > 0.05), and did not differ significantly in the mini-
laparotomy group at all the time (p > 0.05). The rate of peritoneal
metastasis of mouse forestomach
carcinoma was 50% in the
laparotomy group, 45.83% in the mini-
laparotomy group, and 45.45% in the CO(2) insufflation group; this difference was not statistically significant (p > 0.05). The total weight of implanted nodules of mouse forestomach
carcinoma was 1.02 ± 0.38 g in the
laparotomy group, 0.97 ± 0.41 g in the mini-
laparotomy group, and 0.93 ± 0.45 g in the CO(2) insufflation group, which was not a statistically significant difference (p > 0.05).
CONCLUSION: