Abstract | BACKGROUND: METHODS: Nonpregnant ewes were assigned to a control (Con, 100% of National Research Council [NRC] recommendations) or obesogenic (OB, 150% of NRC) diet from 60 days before conception. The large intestine was sampled from fetuses at 135 days (term 150 days) after conception and from offspring lambs at 22.5 ± 0.5 months of age. RESULTS:
Maternal obesity enhanced mRNA expression tumor necrosis factor (TNF)α, interleukin (IL)1α, IL1β, IL6, IL8, and monocyte/macrophage chemotactic protein-1 (MCP1), as well as macrophage markers, CD11b, CD14, and CD68 in fetal gut. mRNA expression of Toll-like receptor (TLR) 2 and TLR4 was increased in OB versus Con fetuses; correspondingly, inflammatory NF-κB and JNK signaling pathways were also upregulated. Both mRNA expression and protein content of transforming growth factor (TGF) β was increased. The IL-17A mRNA expression and protein content was higher in OB compared to Con samples, which was associated with fibrosis in the large intestine of OB fetuses. Similar inflammatory responses and enhanced fibrosis were detected in OB compared to Con offspring. CONCLUSIONS:
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Authors | Xu Yan, Yan Huang, Hui Wang, Min Du, Bret W Hess, Stephen P Ford, Peter W Nathanielsz, Mei-Jun Zhu |
Journal | Inflammatory bowel diseases
(Inflamm Bowel Dis)
Vol. 17
Issue 7
Pg. 1513-22
(Jul 2011)
ISSN: 1536-4844 [Electronic] England |
PMID | 21674707
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, P.H.S.)
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Copyright | Copyright © 2010 Crohn's & Colitis Foundation of America, Inc. |
Chemical References |
- Cytokines
- Interleukin-17
- NF-kappa B
- RNA, Messenger
- Transforming Growth Factor beta
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Topics |
- Animals
- Animals, Newborn
(immunology)
- Blotting, Western
- Cytokines
(genetics, metabolism)
- Female
- Fetus
(immunology, pathology)
- Fibrosis
(etiology, pathology)
- Inflammation
(etiology, pathology)
- Interleukin-17
(genetics, metabolism)
- Intestine, Large
(immunology, metabolism, pathology)
- Maternal Nutritional Physiological Phenomena
- NF-kappa B
(genetics, metabolism)
- Obesity
(complications)
- RNA, Messenger
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Sheep
- Transforming Growth Factor beta
(genetics, metabolism)
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