Increases of vitreous monocyte chemotactic protein 1 and interleukin 8 levels in patients with concurrent hypertension and diabetic retinopathy.

Abstract

PURPOSE:

To investigate whether concurrent hypertension affects vitreous cytokine levels in diabetic retinopathy.

METHODS:

Vitreous samples from 41 patients with diabetic retinopathy with or without concurrent hypertension, who underwent vitrectomy, were collected. Vitreous cytokine concentrations were simultaneously measured using flow cytometry. Patients were stratified according to hypertension or other clinical conditions, and the differences in vitreous levels of monocyte chemotactic protein 1, interleukin 8, vascular endothelial growth factor, interferon-inducible protein 10, and monokine induced by interferon gamma were examined.

RESULTS:

Vitreous levels of monocyte chemotactic protein 1 and interleukin 8 were significantly (P < 0.05) higher in hypertensive patients than in nonhypertensive patients and were significantly (P < 0.05) higher in active diabetic retinopathy than in inactive diabetic retinopathy. Vitreous levels of vascular endothelial growth factor, interferon-inducible protein 10, and monokine induced by interferon gamma were not affected by the coexistence of hypertension. In multivariate models, active diabetic retinopathy (P = 0.004 and P = 0.007), systolic blood pressure (P = 0.039 and P = 0.041), and hypertension (P = 0.032 and P = 0.035) were significant and independent predictors for increased vitreous monocyte chemotactic protein 1 and interleukin 8 levels.

CONCLUSION:

Both monocyte chemotactic protein 1 and interleukin 8 levels were elevated in the vitreous of patients with diabetic retinopathy and concurrent hypertension. These findings may help to explain the epidemiologic and clinical evidence that systemic hypertension exacerbates diabetic retinopathy.

• Authors: Yoshihiro Wakabayashi, Yoshihiko Usui, Yoko Okunuki, Takeshi Kezuka, Masaru Takeuchi, Takuya Iwasaki, Atsushi Ohno, Hiroshi Goto
• Journal: Retina (Philadelphia, Pa.) (Retina) Vol. 31 Issue 9 Pg. 1951-7 (Oct 2011) ISSN: 1539-2864 [Electronic] United States
• PMID: 21610567 (Publication Type: Journal Article)

Chemical References

• CCL2 protein, human
• Chemokine CCL2
• Chemokine CXCL10
• Interleukin-8
• Monokines
• VEGFA protein, human
• Vascular Endothelial Growth Factor A

Topics

• Adult
• Aged
• Chemokine CCL2 (metabolism)
• Chemokine CXCL10 (metabolism)
• Diabetic Retinopathy (complications, metabolism)
• Female
• Fluorescein Angiography
• Humans
• Hypertension (complications, metabolism)
• Immunoassay
• Interleukin-8 (metabolism)
• Male
• Middle Aged
• Monokines (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)
• Vitrectomy
• Vitreous Body (metabolism)