Partially purified extract and viscolin from Viscum coloratum attenuate airway inflammation and eosinophil infiltration in ovalbumin-sensitized mice.

Abstract

AIM OF THE STUDY:

Viscum coloratum Nakai is used in traditional Chinese medicine to treat various diseases, including hemorrhage, hypertension, and inflammatory diseases. A previous study demonstrated a partially purified extract (PPE-SVC) and viscolin from Viscum coloratum Nakai inhibited phosphodiesterase activity. In this study, we evaluated the anti-asthmatic effects of PPE-SVC and viscolin, from Viscum coloratum Nakai, in OVA-sensitized mice.

MATERIALS AND METHODS:

Female BALB/c mice were sensitized and challenged with ovalbumin (OVA). The mice were randomized into groups and treated with PPE-SVC, viscolin, or rolipram by intraperitoneal injection on 1h before each inhalation of OVA and airway hyperresponsiveness (AHR).

RESULTS:

PPE-SVC and viscolin suppressed AHR and reduced eosinophil infiltration of the lungs in OVA-sensitized mice. Moreover, PPE-SVC and viscolin inhibited chemokines, including CCL11 and CCL24, and Th2-associated cytokines in bronchoalveolar lavage fluid. However, PPE-SVC and viscolin could not decrease IL-4, IL-5, and IL-13 levels in cultures of OVA-activated spleen cells.

CONCLUSION:

PPE-SVC and viscolin attenuate airway inflammation and eosinophil infiltration in OVA-sensitized mice.

• Authors: Jiann-Jong Shen, Ming-Shan Chiang, Ming-Ling Kuo, Yann-Lii Leu, Tsong-Long Hwang, Chian-Jiun Liou, Wen-Chung Huang
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 135 Issue 3 Pg. 646-53 (Jun 01 2011) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 21473902 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Asthmatic Agents
• Anti-Inflammatory Agents
• Biphenyl Compounds
• Chemokines
• Cytokines
• Plant Extracts
• viscolin
• Ovalbumin
• Rolipram
• Propane

Topics

• Animals
• Anti-Asthmatic Agents (isolation & purification, pharmacology, therapeutic use)
• Anti-Inflammatory Agents (isolation & purification, pharmacology, therapeutic use)
• Biphenyl Compounds (isolation & purification, pharmacology, therapeutic use)
• Bronchial Hyperreactivity (immunology, metabolism, prevention & control)
• Bronchoalveolar Lavage Fluid (chemistry)
• Chemokines (metabolism)
• Cytokines (metabolism)
• Eosinophils (metabolism)
• Female
• Inflammation (immunology, metabolism, prevention & control)
• Lung (drug effects, immunology)
• Medicine, Chinese Traditional
• Mice
• Mice, Inbred BALB C
• Ovalbumin
• Phytotherapy
• Plant Extracts (chemistry, pharmacology, therapeutic use)
• Propane (analogs & derivatives, isolation & purification, pharmacology, therapeutic use)
• Random Allocation
• Rolipram
• Spleen (cytology, drug effects)
• Viscum (chemistry)