Genetic predisposition to persistent apical periodontitis.

Abstract

INTRODUCTION:

The proinflammatory cytokine interleukin (IL)-1 is a key regulator of host responses to microbial infection and a major modulator of extracellular matrix catabolism and bone resorption. Allele2 of IL-1β is associated with a four-fold increase in IL-1β production. The aim of this case-control study was to evaluate the gene polymorphism of IL-1β in the pathogenesis of endodontic failure. We hypothesized that the gene polymorphism (allele2 of IL-1β) would influence host response and enhance inflammatory reactions predisposing to persistent apical periodontitis (PAP).

MATERIALS AND METHODS:

Subjects with at least 1 year of follow-up after root canal therapy (RCT) were recalled. Inclusion and exclusion criteria were applied, and 34 subjects with signs/symptoms of PAP with otherwise acceptable RCT were included. Sixty-one controls showed healing with acceptable RCT. Genomic DNA from buccal mucosa was amplified by polymerase chain reaction followed by restriction fragment length polymorphism to distinguish the alleles of IL-1β gene polymorphism.

RESULTS:

A significant difference in the distribution of the polymorphic genotype among cases (70.6%) and controls (24.6%) (P < .001, Pearson χ(2)) was shown.

CONCLUSIONS:

These findings suggest that specific genetic markers associated with increased IL-1β production may contribute to increased susceptibility to PAP.

• Authors: Jussara M Morsani, Anita Aminoshariae, Yiping Weng Han, Thomas A Montagnese, Andre Mickel
• Journal: Journal of endodontics (J Endod) Vol. 37 Issue 4 Pg. 455-9 (Apr 2011) ISSN: 1878-3554 [Electronic] United States
• PMID: 21419289 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Chemical References

• Inflammation Mediators
• Interleukin-1beta

Topics

• Alleles
• Case-Control Studies
• Female
• Follow-Up Studies
• Genetic Predisposition to Disease (genetics)
• Genotype
• Host-Pathogen Interactions (genetics)
• Humans
• Inflammation Mediators
• Interleukin-1beta (genetics)
• Male
• Middle Aged
• Periapical Periodontitis (diagnostic imaging, genetics)
• Polymorphism, Genetic (genetics)
• Polymorphism, Restriction Fragment Length (genetics)
• Radiography
• Recurrence
• Retrospective Studies
• Root Canal Therapy
• Tooth, Nonvital (diagnostic imaging)
• Treatment Failure