Protective effect of a polyphenolic rich extract from Magnolia officinalis bark on influenza virus-induced pneumonia in mice.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Magnolia officinalis bark is used in traditional Chinese medicine for the treatment of cough, colds, fever, chronic bronchitis and stomach ailments.

AIM OF THE STUDY:

To investigate therapeutic effects of polyphenol rich extract from M. officinalis bark (MPE) in influenza virus A-infected mice, and to provide evidence for the inflammation response and immunomodulatory potential during infection.

MATERIALS AND METHODS:

Mice were infected with influenza virus A (IVA) and MPE at doses of 10 and 20mg/kg were orally administrated daily for 5 days after challenge. The levels of serum L-6 and TNF-α were determined by ELISA while protein expressions of NF-κB and TLR3 were detected by western blotting analysis.

RESULTS:

MPE exhibited significant therapeutical effects on reducing levels of serum NO, IL-6 and TNF-α, inhibiting pneumonia, decreasing lung viral titers and sensitizing IVA-induced apoptosis through down-regulation of NF-κB and TLR3 protein expression in the lung tissue of IVA-infected mice.

CONCLUSIONS:

MPE could exhibit preventive and therapeutical effects on IVA-infected mice as a suppressor of the production of inflammatory mediators, NO and pro-inflammatory cytokines, TNF-α and IL-6. These effects appeared to be mediated, at least in part, by an inhibition of TLR3 and NF-κB activation. Therefore, MPE could provide a safe and effective therapeutic approach for influenza and its subsequent viral pneumonia.

• Authors: Xiao-Ning Wu, Chen-Huan Yu, Wei Cai, Jian Hua, Shi-Qing Li, Wei Wang
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 134 Issue 1 Pg. 191-4 (Mar 08 2011) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 21146600 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Flavonoids
• NF-kappa B
• Phenols
• Plant Extracts
• Polyphenols
• Toll-Like Receptor 3

Topics

• Animals
• Blotting, Western
• Enzyme-Linked Immunosorbent Assay
• Flavonoids (therapeutic use)
• Influenza A virus (isolation & purification)
• Magnolia (chemistry)
• Mice
• Mice, Inbred ICR
• NF-kappa B (metabolism)
• Phenols (therapeutic use)
• Plant Bark (chemistry)
• Plant Extracts (therapeutic use)
• Pneumonia, Viral (prevention & control, virology)
• Polyphenols
• Toll-Like Receptor 3 (metabolism)