Abstract |
Human herpesvirus 6 (HHV-6) has a tropism for immunocompetent cells, including T lymphocytes, monocytes/macrophages, and dendritic cells (DCs) suggesting that HHV-6 infection affects the immunosurveillance system. Toll-like receptor (TLR) system plays an important role in innate immunity against various pathogens. In the present study, we investigated the effect of HHV-6 infection on the expression and intracellular signaling of TLRs in DCs. Although expression levels of TLRs were not decreased or slightly elevated following HHV-6 infection, the amounts of cytokines produced following stimulation with ligands for TLRs appeared to be dramatically decreased in HHV-6-infected DCs as compared to mock-infected DCs. Similarly, phosphorylation levels of TAK-1, IkappaB kinase, and IkappaB-alpha following stimulation of HHV-6-infected DCs with lipopolysaccharide, which is the ligand for TLR4, appeared to be decreased. These data show that HHV-6 impairs intracellular signaling through TLRs indicating the novel mechanism of HHV-6-mediated immunomodulation.
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Authors | Yuichi Murakami, Kazushi Tanimoto, Hiroshi Fujiwara, Jun An, Koichiro Suemori, Toshiki Ochi, Hitoshi Hasegawa, Masaki Yasukawa |
Journal | Virology journal
(Virol J)
Vol. 7
Pg. 91
(May 10 2010)
ISSN: 1743-422X [Electronic] England |
PMID | 20459723
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Toll-Like Receptors
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Topics |
- Adult
- Cells, Cultured
- Cytokines
(metabolism)
- Dendritic Cells
(virology)
- Gene Expression Profiling
- Herpesvirus 6, Human
(immunology, pathogenicity)
- Humans
- Signal Transduction
- Toll-Like Receptors
(immunology)
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