Clinical relevance of 13 cytokine gene polymorphisms in Chinese major trauma patients.

Abstract

PURPOSE:

To determine the clinical relevance of 13 reported common single-nucleotide polymorphisms (SNPs) of cytokine genes in patients with major trauma.

METHODS:

Thirteen SNPs in nine key cytokine genes were selected for association study in 308 patients with major trauma on the basis of previous functional or association data. An allele-specific oligonucleotide array was developed and used to genotype 308 patients with major trauma. The clinical relevance of the 13 SNPs was assessed by observation of cytokine production and outcome of trauma patients.

RESULTS:

Results from the allele-specific oligonucleotide array indicated that 8 [interleukin-1beta (IL-1beta)/-1470, IL-1beta/-511, IL-1beta/-31, IL-4/-589, IL-6/-572, IL-8/-251, IL-10/-819, and tumor necrosis factor alpha (TNFalpha)/-308] out of the 13 SNPs were associated with respective ex vivo cytokine production by peripheral leukocytes in response to lipopolysaccharide (LPS) stimulation at admission, or risk of development of sepsis or organ dysfunction in major trauma patients. Patients with more than four risk alleles of the eight SNPs had more than 50% sepsis morbidity and more severe organ dysfunction.

CONCLUSIONS:

Polymorphisms of IL-1beta/-1470, IL-1beta/-511, IL-1beta/-31, IL-4/-589, IL-6/-572, IL-8/-251, IL-10/-819, and TNFalpha/-308 are susceptibility loci for the development of sepsis and organ dysfunction in major trauma patients.

• Authors: Wei Gu, Ling Zeng, Jian Zhou, Dong-po Jiang, Lianyang Zhang, Ding-yuan Du, Ping Hu, Kehong Chen, Qin Liu, Zheng-guo Wang, Jian-xin Jiang
• Journal: Intensive care medicine (Intensive Care Med) Vol. 36 Issue 7 Pg. 1261-5 (Jul 2010) ISSN: 1432-1238 [Electronic] United States
• PMID: 20217044 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines

Topics

• Adult
• Asian People (genetics)
• China
• Cytokines (genetics)
• Female
• Gene Expression Profiling
• Humans
• Male
• Multiple Organ Failure (genetics)
• Multiple Trauma (genetics)
• Oligonucleotide Array Sequence Analysis
• Polymorphism, Single Nucleotide
• Prospective Studies
• Sepsis (genetics)
• Trauma Severity Indices