Abstract | BACKGROUND: METHODS: In a longitudinal investigation, we explored patterns of lineage specific and immaturity-associated antigens in T-ALL in a large cohort of patients treated under the multicenter Children's Oncology Group (COG) protocol. All samples were analyzed using multicolor flow cytometry in a standardized fashion at a single institution. RESULTS: We report that markers of immaturity particularly, TdT and CD99, dramatically decline on leukemic blasts during therapy. CD34 and CD10 expression is confined to a minority of pretreatment samples and is also not stable. In contrast, lineage-associated markers including CD2, CD3, CD4, CD5, CD7, and CD8 failed to show significant trends. CONCLUSIONS: Our study strongly argues for expansion of immunophenotyping panels for T-ALL MRD to decrease reliance on immature antigens. This study represents the first demonstration of consistent immunophenotypic shifts in T-ALL.
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Authors | Mikhail Roshal, Jonathan R Fromm, Stuart Winter, Kimberly Dunsmore, Brent L Wood |
Journal | Cytometry. Part B, Clinical cytometry
(Cytometry B Clin Cytom)
Vol. 78
Issue 3
Pg. 139-46
(May 2010)
ISSN: 1552-4957 [Electronic] United States |
PMID | 20155852
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | 2010 Clinical Cytometry Society. |
Chemical References |
- Antigens, CD
- Biomarkers, Tumor
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Topics |
- Antigens, CD
(analysis, immunology)
- Biomarkers, Tumor
(analysis, immunology)
- Cohort Studies
- Flow Cytometry
- Humans
- Longitudinal Studies
- Neoplasm, Residual
(diagnosis, immunology)
- Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
(diagnosis, immunology)
- Reproducibility of Results
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