Analysis of gene expression in mouse alveolar macrophages stimulated with quorum-sensing mutants of Vibrio vulnificus.

Abstract	Bacterial pathogens manipulate host cells to promote pathogen survival and dissemination. In this study, microarray technology was used to identify the genes that are affected by the Vibrio vulnificus quorum-sensing genes, luxS and smcR. By comparing the expression profiles of mouse macrophage cell lines stimulated with either the parent strains or a luxS smcR mutant, differentially expressed genes were identified. The genes included those that affect host cell death, stress, signaling transduction, inflammation, and immune response. Macrophages stimulated with the luxS smcR mutant differentially expressed genes associated with removal of toxins, the complement pathway, regulation of cytokine expression, and antigen presentation, indicating that macrophages stimulated with the luxS smcR mutant induced an appropriate inflammation reaction and immune response for removal of bacteria. In summary, quorum-sensing in V. vulnificus could contribute to bacterial survival and increased pathogenesis by inducing a changed expression profile in macrophages.
Authors	Na-Ri Shin, Deog-Yong Lee, Han Sang Yoo
Journal	Japanese journal of infectious diseases (Jpn J Infect Dis) Vol. 61 Issue 5 Pg. 402-6 (Sep 2008) ISSN: 1344-6304 [Print] Japan
PMID	18806354 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Bacterial Proteins Proteins Trans-Activators smcR protein, Vibrio vulnificus Carbon-Sulfur Lyases LuxS protein, Bacteria
Topics	Animals Bacterial Proteins (genetics, metabolism) Carbon-Sulfur Lyases (genetics, metabolism) Cell Line Gene Expression Profiling Gene Expression Regulation Macrophages, Alveolar (microbiology) Mice Mutation Oligonucleotide Array Sequence Analysis Proteins (genetics, metabolism) Quorum Sensing Trans-Activators (genetics, metabolism) Vibrio vulnificus (genetics, growth & development, metabolism, pathogenicity)

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