Attenuation of lipopolysaccharide-induced inflammatory response and phospholipids metabolism at the feto-maternal interface by N-acetyl cysteine.

Abstract

Maternal microbial infections cause adverse fetal developmental outcomes including embryonic resorption, intrauterine fetal death, and preterm labor. Recent studies demonstrated that oxidative-stress plays an important role in chorioamniotitis pathogenesis. Herein we investigated the effect of N-acetyl cysteine (NAC) on lipopolysaccharide (LPS)-induced preterm labor and fetal demise in murine model. Lipopolysaccharide exposure at embryonic day 18 demonstrated an increase in the abortion rate and fetal demise in pregnant rats. This was associated with increase in an inflammatory response (cytokines, chemokines, and iNOS expression) and infiltration of leukocytes (monocytes and polymorphonuclear cells) in the placenta. There was increased expression of cytosolic and secretary phospholipase A2 with increased secretion of prostaglandin-2 and leukotriene B4 in the placenta, suggestive of increased metabolism of phospholipids. In addition, expression of cycloxygenase-2 and malondialdehyde production (oxidative-stress marker) was increased in the placenta. Conversely, NAC pretreatment abolished these effects of LPS in the placenta. Collectively, these data provide evidence that LPS-induced increased inflammation and metabolism of phospholipids at the feto-maternal interface (placenta) is critical for preterm labor and fetal demise during maternal microbial infections which could be blocked by antioxidant-based therapies.

• Authors: Manjeet K Paintlia, Ajaib S Paintlia, Avtar K Singh, Inderjit Singh
• Journal: Pediatric research (Pediatr Res) Vol. 64 Issue 4 Pg. 334-9 (Oct 2008) ISSN: 1530-0447 [Electronic] United States
• PMID: 18552708 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• DNA Primers
• Lipopolysaccharides
• Phospholipids
• Malondialdehyde
• Cyclooxygenase 2
• Acetylcysteine

Topics

• Acetylcysteine (therapeutic use)
• Animals
• Cyclooxygenase 2 (metabolism)
• DNA Primers (genetics)
• Female
• Immunoblotting
• Immunoenzyme Techniques
• Inflammation (drug therapy, immunology, microbiology)
• Lipid Peroxidation (physiology)
• Lipopolysaccharides (toxicity)
• Malondialdehyde (metabolism)
• Maternal-Fetal Exchange (physiology)
• Phospholipids (metabolism)
• Placenta (immunology, metabolism, pathology)
• Pregnancy
• Rats
• Reverse Transcriptase Polymerase Chain Reaction