Involvement of retinoic acid-inducible gene-I in inflammation of rheumatoid fibroblast-like synoviocytes.

Abstract

Interferon (IFN)-gamma is a major cytokine that regulates T helper 1-type immune reactions and serves as an important mediator in the pathogenesis of autoimmune diseases. Retinoic acid-inducible gene-I (RIG-I) is an IFN-gamma-inducible gene and known to be involved in the inflammatory and immune reactions. In the present study, we found high levels of RIG-I expression in synovial tissues of rheumatoid arthritis (RA), while the expression in osteoarthritis tissues was low. Treatment of cultured fibroblast-like synoviocytes with IFN-gamma markedly induced the expression of RIG-I. Knockdown of RIG-I in fibroblast-like synoviocytes, with specific siRNA, resulted in the inhibition of the IFN-gamma-induced expression of chemokine (C-X-C motif) ligand 10 (CXCL10)/IFN-gamma-inducible protein-10 (IP-10), a chemokine with chemotactic activity towards T cells. These findings suggest that RIG-I may play an important role in the pathogenesis of synovial inflammation in RA, at least in part, by regulating the IFN-gamma-induced expression of CXCL10/IP-10.

• Authors: T Imaizumi, T Arikawa, T Sato, R Uesato, T Matsumiya, H Yoshida, M Ueno, S Yamasaki, T Nakajima, M Hirashima, K Sakata, Y Ishibashi, S Toh, C Ohyama, K Satoh
• Journal: Clinical and experimental immunology (Clin Exp Immunol) Vol. 153 Issue 2 Pg. 240-4 (Aug 2008) ISSN: 1365-2249 [Electronic] England
• PMID: 18505427 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Chemokine CXCL10
• RNA, Messenger
• RNA, Small Interfering
• Receptors, Immunologic
• Interferon-gamma
• RIGI protein, human
• DEAD Box Protein 58
• DEAD-box RNA Helicases

Topics

• Arthritis, Rheumatoid (immunology, metabolism)
• Cells, Cultured
• Chemokine CXCL10 (metabolism)
• Chemotaxis, Leukocyte
• DEAD Box Protein 58
• DEAD-box RNA Helicases (analysis, genetics)
• Fibroblasts (immunology, metabolism)
• Gene Expression (drug effects)
• Humans
• Immunohistochemistry
• Interferon-gamma (pharmacology)
• Osteoarthritis (immunology)
• RNA Interference
• RNA, Messenger (analysis)
• RNA, Small Interfering (pharmacology)
• Receptors, Immunologic
• Synovial Membrane (immunology, metabolism)