Abstract | RATIONALE: OBJECTIVES: The aim of this study was to identify the initiating lesions and investigate the natural progression of lung disease caused by airway surface dehydration. METHODS: Lung morphology, gene expression, bronchoalveolar lavage, and lung mechanics were studied at different ages in betaENaC-overexpressing mice. MEASUREMENTS AND MAIN RESULTS: Mucus obstruction in betaENaC-overexpressing mice originated in the trachea in the first days of life and was associated with hypoxia, airway epithelial necrosis, and death. In surviving betaENaC-overexpressing mice, mucus obstruction extended into the lungs and was accompanied by goblet cell metaplasia, increased mucin expression, and airway inflammation with transient perinatal increases in tumor necrosis factor-alpha and macrophages, IL-13 and eosinophils, and persistent increases in keratinocyte-derived cytokine (KC), neutrophils, and chitinases in the lung. betaENaC-overexpressing mice also developed emphysema with increased lung volumes, distal airspace enlargement, and increased lung compliance. CONCLUSIONS:
|
Authors | Marcus A Mall, Jack R Harkema, Joanna B Trojanek, Diana Treis, Alessandra Livraghi, Susanne Schubert, Zhe Zhou, Silvia M Kreda, Stephen L Tilley, Elizabeth J Hudson, Wanda K O'Neal, Richard C Boucher |
Journal | American journal of respiratory and critical care medicine
(Am J Respir Crit Care Med)
Vol. 177
Issue 7
Pg. 730-42
(Apr 01 2008)
ISSN: 1535-4970 [Electronic] United States |
PMID | 18079494
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Epithelial Sodium Channels
|
Topics |
- Animals
- Animals, Newborn
- Bronchitis, Chronic
(physiopathology)
- Dehydration
- Disease Progression
- Epithelial Sodium Channels
(metabolism)
- Gene Expression
- Inflammation
- Longitudinal Studies
- Mice
- Mice, Inbred Strains
- Mucociliary Clearance
- Pulmonary Disease, Chronic Obstructive
(physiopathology)
- Pulmonary Emphysema
(physiopathology)
- Respiratory Mucosa
(metabolism)
|