Cytokine modulation of retinoic acid-inducible gene-I (RIG-I) expression in human epidermal keratinocytes.

Abstract

BACKGROUND:

Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH/D box family proteins and designated as a putative RNA helicase, which plays various roles in gene expression and cellular functions in response to a variety of RNA viruses.

OBJECTIVE:

The present study was designed to investigate the effects of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha on RIG-I expression in human keratinocytes, and the expression of RIG-I in skin lesions of psoriasis vulgaris, in which IFN-gamma and TNF-alpha are considered to be involved in its pathogenesis.

METHODS:

The mRNA and protein expression of RIG-I was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Immunohistochemical staining of RIG-I was examined on psoriatic skin section.

RESULTS:

The levels of RIG-I mRNA and protein were upregulated in HaCaT keratinocytes in the presence of IFN-gamma or TNF-alpha. Immunohistochemically, RIG-I was detected in the cytoplasm of the spinous and basal layers of psoriatic skin.

CONCLUSION:

Our results suggest that RIG-I might operate not only as a RNA helicase but also as a mediator of the cytokine network in the inflammatory skin diseases, such as psoriasis vulgaris.

• Authors: Hideo Kitamura, Yasushi Matsuzaki, Kazuyuki Kimura, Hajime Nakano, Tadaatsu Imaizumi, Kei Satoh, Katsumi Hanada
• Journal: Journal of dermatological science (J Dermatol Sci) Vol. 45 Issue 2 Pg. 127-34 (Feb 2007) ISSN: 0923-1811 [Print] Netherlands
• PMID: 17182220 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• Receptors, Immunologic
• Tumor Necrosis Factor-alpha
• Interferon-gamma
• RIGI protein, human
• DEAD Box Protein 58
• DEAD-box RNA Helicases
• RNA Helicases

Topics

• Adult
• Aged
• Biopsy
• Cell Line, Transformed
• Cytoplasm (metabolism)
• DEAD Box Protein 58
• DEAD-box RNA Helicases (genetics, metabolism)
• Epidermal Cells
• Gene Expression (drug effects)
• Humans
• Interferon-gamma (metabolism, pharmacology)
• Keratinocytes (cytology, physiology)
• Middle Aged
• Psoriasis (pathology, physiopathology)
• RNA Helicases (genetics, metabolism)
• RNA, Messenger (metabolism)
• Receptors, Immunologic
• Tumor Necrosis Factor-alpha (metabolism, pharmacology)