Abstract | BACKGROUND: METHOD:
Formate dehydrogenase-loaded erythrocytes were prepared by hypotonic dialysis method. Carbicarb (carb) (equimolar solution of sodium carbonate and sodium bicarbonate) was used to treat metabolic acidosis. Folate depletion was induced by methotrexate (MTX) treatment. Experimental design consisted of 8 groups: saline control, methanol control, MTX control, ELE control, MTX- methanol control, MTX- methanol-carb, MTX- methanol-carb-ELE, and MTX-MeOH-ELE group. Male Wistar rats treated with MTX (0.3 mg/kg) for a week were injected (i.p.) with methanol (4 g/kg). Twelve hours later, the carbicarb solution was infused, and then a formate dehydrogenase-loaded erythrocytes suspension (40% hematocrit) was infused (i.v.) in bolus. Blood samples were collected every hour for 4 h from the cannulated left jugular vein. Blood methanol and formate were estimated respectively with HPLC and fluorimetric assay. Blood pH, blood pO2, pCO2 and bicarbonate were also measured. RESULTS: There was marked elimination of formate in selected groups. CONCLUSION:
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Authors | Arumugham Muthuvel, Rathinam Rajamani, Sundaramahalingam Manikandan, Rathinasamy Sheeladevi |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 367
Issue 1-2
Pg. 162-9
(May 2006)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 16445901
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carbonates
- Drug Combinations
- Formates
- sodium bicarbonate, sodium carbonate drug combination
- Sodium Bicarbonate
- Formate Dehydrogenases
- Methanol
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Topics |
- Animals
- Carbonates
(blood, pharmacology)
- Dialysis
- Drug Combinations
- Erythrocytes
(drug effects, enzymology)
- Formate Dehydrogenases
(metabolism)
- Formates
(blood, toxicity)
- Hydrogen-Ion Concentration
- Male
- Methanol
(poisoning)
- Rats
- Rats, Wistar
- Sodium Bicarbonate
(blood, pharmacology)
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