Effect of anticancer agents on codeinone-induced apoptosis in human cancer cell lines.
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| Abstract |
The possible apoptosis-inducing activity of codeinone, an oxidative metabolite of codeine, without or with anticancer drugs, was investigated. Codeinone induced internucleosomal DNA fragmentation in human promyelocytic leukemia cells (HL-60), but not in human squamous cell carcinoma cells (HSC-2). Codeinone dose-dependently activated caspase-3 in both of these cells, but to a much lesser extent than that attained by actinomycin D. This property of codeinone was similar to what we have found previously in alpha,beta-unsaturated ketones. Codeinone did not activate caspase-8 or caspase-9 in these cells. The cytotoxic activity of codeinone against HSC-2 cells was inhibited by N-acetyl-L-cysteine, but somewhat additively stimulated by sodium ascorbate, epigallocatechin gallate, hydrogen peroxide, sodium fluoride, 5-fluorouridine, cisplatin, doxorubicin and methotrexate. These data suggest that codeinone has possible antitumor potential, in addition to its action as a narcotic analgesic, even though it induces incomplete apoptosis-associated characteristics.
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| Authors | Risa Takeuchi, Hiroshi Hoshijima, Noriko Onuki, Hiroshi Nagasaka, Shahead Ali Chowdhury, Masami Kawase, Hiroshi Sakagami |
| Journal | Anticancer research (Anticancer Res) 2005 Nov-Dec Vol. 25 Issue 6B Pg. 4037-41 ISSN: 0250-7005 [Print] Greece |
| PMID | 16309196 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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